PMID- 20179892
OWN - NLM
STAT- MEDLINE
DCOM- 20130124
LR  - 20181201
IS  - 0371-0874 (Print)
IS  - 0371-0874 (Linking)
VI  - 62
IP  - 1
DP  - 2010 Feb 25
TI  - [Rapid differentiation of human umbilical cord-derived mesenchymal stem cells 
      into insulin-secreting cells under the sole induction of biological products].
PG  - 73-8
AB  - In order to explore the feasibility of inducing the human umbilical cord-derived 
      mesenchymal stem cells (hUC-MSCs) to differentiate into insulin-secreting cells 
      with biological products alone, hUC-MSCs were separated and purified from the 
      whole umbilical cord by the sequent digestion of collagenase II and trypsin 
      followed by two-step centrifugation. hUC-MSCs were induced with IMDM culture 
      medium containing epidermal growth factor (EGF), basic fibroblast growth factor 
      (bFGF) and Ginkgo biloba extract (GBE). Before and after the induction, the 
      morphological changes were observed under inverse microscope; the islet-related 
      genes were detected by RT-PCR; islet-like clusters (ILCs) were identified by 
      dithizone (DTZ) staining; PDX-1 and immunoreactive insulin (IRI) were examined by 
      immunofluorescence method; the quantity and quality of IRI secretion were assayed 
      by chemiluminescence immunoassay and Western blot respectively. The results 
      showed that the purified hUC-MSCs presented long spindle-like shape and parallel 
      or spiral arrangement which are typical morphological features of MSCs. After the 
      induction, hUC-MSCs changed gradually into round or oval shape and gathered 
      together to form ILCs; there were more than one hundred clusters on the growth 
      surface of a flask of T25; ILCs were stained into positive mauve by DTZ and 
      positive for PDX-1 and IRI; Western blot displayed that most of the IRI was 
      proinsulin (PI). Therefore, hUC-MSCs can rapidly differentiate into 
      insulin-secreting cells under the sole induction of EGF, bFGF, GBE and IMDM, but 
      ILCs are not mature enough to produce sufficient true insulin.
FAU - Wang, Yue-Chun
AU  - Wang YC
AD  - Department of Physiology; Institute of Hematology, Jinan University Medical 
      College, Guangzhou 510632, China. monkeymei2@yahoo.com.cn
FAU - Zhang, Yuan
AU  - Zhang Y
FAU - Duan, A-Lin
AU  - Duan AL
FAU - Lin, Wei-Xia
AU  - Lin WX
FAU - Zheng, Qiao-Dan
AU  - Zheng QD
FAU - Xu, Wen-Lu
AU  - Xu WL
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Li Xue Bao
JT  - Sheng li xue bao : [Acta physiologica Sinica]
JID - 20730130R
RN  - 0 (Plant Extracts)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 62229-50-9 (Epidermal Growth Factor)
SB  - IM
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Epidermal Growth Factor/pharmacology
MH  - Fibroblast Growth Factor 2/pharmacology
MH  - Ginkgo biloba/chemistry
MH  - Humans
MH  - Insulin-Secreting Cells/*cytology
MH  - Mesenchymal Stem Cells/*cytology
MH  - Plant Extracts/pharmacology
MH  - Umbilical Cord/*cytology
EDAT- 2010/02/25 06:00
MHDA- 2013/01/25 06:00
CRDT- 2010/02/25 06:00
PHST- 2010/02/25 06:00 [entrez]
PHST- 2010/02/25 06:00 [pubmed]
PHST- 2013/01/25 06:00 [medline]
PST - ppublish
SO  - Sheng Li Xue Bao. 2010 Feb 25;62(1):73-8.