PMID- 2018524
OWN - NLM
STAT- MEDLINE
DCOM- 19910520
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 176
IP  - 1
DP  - 1991 Apr 15
TI  - Characterization of monocyte chemotactic protein-1 binding to human monocytes.
PG  - 309-14
AB  - Monocyte chemotactic protein-1 (MCP-1) stimulates chemotaxis of peripheral blood 
      monocytes. In order to understand the biologic basis of this specific activity, 
      binding studies of 125I-MCP-1 were undertaken. MCP-1 showed saturable binding to 
      monocytes. Scatchard analysis of the monocyte binding data indicate that there 
      are approximately 1,600 high affinity binding sites per monocyte with a Kd = 1.1 
      nM. Studies with synthetic peptides constructed according to the MCP-1 amino acid 
      sequence indicate that a synthetic peptide, MCP-1[13-35], stimulates monocyte 
      migration and competes with native MCP-1 for binding sites. Inhibition of MCP-1 
      binding was tested with chemotactic connective tissue proteins. No inhibition of 
      MCP-1 binding was observed with either collagen, elastin-derived peptides or 
      fibronectin. These results identify a single class of unique high affinity MCP-1 
      binding sites that are likely to recognize a peptide domain on MCP-1 which 
      include the amino acids within the region, 13-35.
FAU - Valente, A J
AU  - Valente AJ
AD  - Department of Pathology, University of Texas Health Science Center, San Antonio 
      78284.
FAU - Rozek, M M
AU  - Rozek MM
FAU - Schwartz, C J
AU  - Schwartz CJ
FAU - Graves, D T
AU  - Graves DT
LA  - eng
GR  - HL-07446/HL/NHLBI NIH HHS/United States
GR  - HL-26890/HL/NHLBI NIH HHS/United States
GR  - HL-38390/HL/NHLBI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Fibronectins)
RN  - 0 (Peptide Fragments)
RN  - 9007-34-5 (Collagen)
RN  - 9007-58-3 (Elastin)
SB  - IM
MH  - Animals
MH  - Anti-Infective Agents/*metabolism
MH  - Binding, Competitive
MH  - Chemokine CCL2
MH  - Chemotactic Factors/isolation & purification/*metabolism/pharmacology
MH  - Chemotaxis, Leukocyte/drug effects
MH  - Collagen/pharmacology
MH  - Elastin/pharmacology
MH  - Fibronectins/pharmacology
MH  - Humans
MH  - In Vitro Techniques
MH  - Kinetics
MH  - Leukocytes, Mononuclear/*physiology
MH  - Muscle, Smooth, Vascular/chemistry
MH  - Papio
MH  - Peptide Fragments/pharmacology
MH  - Protein Binding
EDAT- 1991/04/15 00:00
MHDA- 1991/04/15 00:01
CRDT- 1991/04/15 00:00
PHST- 1991/04/15 00:00 [pubmed]
PHST- 1991/04/15 00:01 [medline]
PHST- 1991/04/15 00:00 [entrez]
AID - 0006-291X(91)90925-W [pii]
AID - 10.1016/0006-291x(91)90925-w [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1991 Apr 15;176(1):309-14. doi: 
      10.1016/0006-291x(91)90925-w.