PMID- 20186656
OWN - NLM
STAT- MEDLINE
DCOM- 20110208
LR  - 20131121
IS  - 1439-0221 (Electronic)
IS  - 0032-0943 (Linking)
VI  - 76
IP  - 10
DP  - 2010 Jul
TI  - The potential mechanism of tiliroside-dependent inhibition of 
      t-butylhydroperoxide-induced oxidative stress in endometrial carcinoma cells.
PG  - 963-8
LID - 10.1055/s-0029-1240900 [doi]
AB  - The effects of oxidative stress on collagen and DNA biosynthesis, 
      beta-galactosidase activity, the expression of the beta-integrin receptor, FAK, 
      the insulin-like growth factor-I receptor (IGF-IR), the hypoxia-inducible 
      factor-1 (HIF-1), and the mitogen-activated protein kinases (MAP/ERK(1), ERK(2)) 
      were evaluated in human endometrial carcinoma cells. Subconfluent cells were 
      subjected to oxidative stress with 30 microM t-butylhydroperoxide (t-BHP) for 1 h 
      per day over the course of 5 days. It was found that oxidative stress contributed 
      to an increase in the beta-galactosidase activity as well as to the inhibition of 
      collagen and DNA biosynthesis. The mechanism of the process was found at the 
      level of IGF-IR and HIF-1 alpha. An increase in the expression of HIF-1 alpha and 
      a decrease in the expression of IGF-IR were observed in the cells subjected to 
      oxidative stress. The role of IGF-IR signalling in the process was confirmed by 
      an experiment showing downregulation of MAP kinases ERK(1) and ERK(2) expression 
      in the studied cells. This phenomenon is probably responsible for the drastic 
      inhibition of protein (up to 40 % of control) and DNA biosynthesis (up to 65 % of 
      control) in the cells. An addition of tiliroside to the cells medium restored all 
      parameters to the control level, including IGF-IR and HIF-1 alpha expressions. 
      The data suggest that the antioxidative activity of tiliroside isolated from 
      Potentilla argentea may originate at the level of IGF-IR and HIF-1 alpha 
      signalling.
CI  - Georg Thieme Verlag KG Stuttgart.New York.
FAU - Tomczyk, Michal
AU  - Tomczyk M
AD  - Department of Pharmacognosy, Faculty of Pharmacy, Medical University of 
      Bialystok, 15-089 Bialystok, Poland.
FAU - Tumanov, Aleksander
AU  - Tumanov A
FAU - Zaniewska, Agnieszka
AU  - Zaniewska A
FAU - Surazynski, Arkadiusz
AU  - Surazynski A
LA  - eng
PT  - Journal Article
DEP - 20100225
PL  - Germany
TA  - Planta Med
JT  - Planta medica
JID - 0066751
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Antioxidants)
RN  - 0 (Flavonoids)
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (Insulin-Like Growth Factor Binding Protein 1)
RN  - 0 (Plant Extracts)
RN  - 15M04TXR9M (tiliroside)
RN  - 9007-34-5 (Collagen)
RN  - 9007-49-2 (DNA)
RN  - 955VYL842B (tert-Butylhydroperoxide)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Antineoplastic Agents, Phytogenic/isolation & 
      purification/*pharmacology/therapeutic use
MH  - Antioxidants/isolation & purification/*pharmacology/therapeutic use
MH  - Cell Line, Tumor
MH  - Collagen/biosynthesis
MH  - DNA/biosynthesis
MH  - Down-Regulation
MH  - Endometrial Neoplasms/drug therapy/*metabolism
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Female
MH  - Flavonoids/isolation & purification/*pharmacology/therapeutic use
MH  - Humans
MH  - Hypoxia-Inducible Factor 1/metabolism
MH  - Insulin-Like Growth Factor Binding Protein 1/metabolism
MH  - Oxidative Stress/*drug effects
MH  - Phytotherapy
MH  - Plant Components, Aerial
MH  - Plant Extracts/chemistry/*pharmacology/therapeutic use
MH  - Potentilla/*chemistry
MH  - Signal Transduction/drug effects
MH  - beta-Galactosidase/metabolism
MH  - tert-Butylhydroperoxide
EDAT- 2010/02/27 06:00
MHDA- 2011/02/09 06:00
CRDT- 2010/02/27 06:00
PHST- 2010/02/27 06:00 [entrez]
PHST- 2010/02/27 06:00 [pubmed]
PHST- 2011/02/09 06:00 [medline]
AID - 10.1055/s-0029-1240900 [doi]
PST - ppublish
SO  - Planta Med. 2010 Jul;76(10):963-8. doi: 10.1055/s-0029-1240900. Epub 2010 Feb 25.