PMID- 20187116
OWN - NLM
STAT- MEDLINE
DCOM- 20100820
LR  - 20220311
IS  - 1554-527X (Electronic)
IS  - 0736-0266 (Linking)
VI  - 28
IP  - 9
DP  - 2010 Sep
TI  - Extracellular matrix expression of human tenocytes in three-dimensional 
      air-liquid and PLGA cultures compared with tendon tissue: implications for tendon 
      tissue engineering.
PG  - 1170-7
LID - 10.1002/jor.21109 [doi]
AB  - Tenocyte transplantation may prove to be an approach to support healing of tendon 
      defects. Cell-cell and cell-matrix contacts within three-dimensional (3D) 
      cultures may prevent tenocyte dedifferentiation observed in monolayer (2D) 
      culture. The present study compares both neotissue formation and tenocyte 
      extracellular matrix (ECM) expression in 2D and 3D cultures directly with that of 
      native tendon, in order to determine optimal conditions for tendon tissue 
      engineering. Primary human tenocytes were embedded in 
      poly[lactic-co-glycolic-acid] (PLGA)-scaffolds and high-density cultures. 
      Neotissue formation was examined by hematoxyline-eosine (H&E) and 
      immunofluorescence staining. Gene expression of ECM proteins and vascular 
      endothelial growth factor (VEGF) was compared at days 0 (2D), 14, and 28 in 3D 
      cultures and tendon. Histomorphology of 3D culture showed tendon-like tissue as 
      tenocyte cell nuclei became more elongated and ECM accumulated. Type I collagen 
      gene expression was higher in 2D culture than in tendon and decreased in 
      4-week-old 3D cultures, whereas type III collagen was only elevated in 
      high-density culture compared with tendon. Decorin and COMP were reduced in 2D 
      and increased in 3D culture almost to ex vivo level. These results suggest that 
      the 3D high-density or biodegradable scaffolds cultures encourage the 
      differentiation of expanded monolayer tenocytes in vitro to tendon-like tissue.
CI  - (c) 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
FAU - Stoll, Christiane
AU  - Stoll C
AD  - Department of Trauma and Reconstructive Surgery, Charite-University of Medicine, 
      Campus Benjamin Franklin, Berlin, Germany.
FAU - John, Thilo
AU  - John T
FAU - Endres, Michaela
AU  - Endres M
FAU - Rosen, Christian
AU  - Rosen C
FAU - Kaps, Christian
AU  - Kaps C
FAU - Kohl, Benjamin
AU  - Kohl B
FAU - Sittinger, Michael
AU  - Sittinger M
FAU - Ertel, Wolfgang
AU  - Ertel W
FAU - Schulze-Tanzil, Gundula
AU  - Schulze-Tanzil G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Orthop Res
JT  - Journal of orthopaedic research : official publication of the Orthopaedic 
      Research Society
JID - 8404726
RN  - 0 (Biocompatible Materials)
RN  - 0 (Biomarkers)
RN  - 0 (Collagen Type I)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Integrin beta1)
RN  - 0 (VEGFA protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Adult
MH  - Air
MH  - *Biocompatible Materials
MH  - Biomarkers
MH  - Cell Differentiation
MH  - Cell Survival
MH  - Collagen Type I/genetics
MH  - Extracellular Matrix/*physiology
MH  - Extracellular Matrix Proteins/genetics
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Integrin beta1/genetics
MH  - *Lactic Acid
MH  - Male
MH  - Organ Culture Techniques/*methods
MH  - *Polyglycolic Acid
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Tendons/cytology/*physiology/transplantation
MH  - Tissue Engineering/*methods
MH  - Vascular Endothelial Growth Factor A/genetics
EDAT- 2010/02/27 06:00
MHDA- 2010/08/21 06:00
CRDT- 2010/02/27 06:00
PHST- 2010/02/27 06:00 [entrez]
PHST- 2010/02/27 06:00 [pubmed]
PHST- 2010/08/21 06:00 [medline]
AID - 10.1002/jor.21109 [doi]
PST - ppublish
SO  - J Orthop Res. 2010 Sep;28(9):1170-7. doi: 10.1002/jor.21109.