PMID- 20187176
OWN - NLM
STAT- MEDLINE
DCOM- 20100609
LR  - 20211020
IS  - 1522-2594 (Electronic)
IS  - 0740-3194 (Print)
IS  - 0740-3194 (Linking)
VI  - 63
IP  - 3
DP  - 2010 Mar
TI  - Quantitative analysis of hyperpolarized 3He ventilation changes in mice 
      challenged with methacholine.
PG  - 658-66
LID - 10.1002/mrm.22311 [doi]
AB  - The capability to use high-resolution (3)He MRI to depict regional ventilation 
      changes and airway narrowing in mice challenged with methacholine (MCh) offers 
      the opportunity to gain new insights into the study of asthma. However, to fully 
      exploit the value of this novel technique, it is important to move beyond visual 
      inspection of the images toward automated and quantitative analysis. To address 
      this gap, we describe a postprocessing approach to create ventilation difference 
      maps to better visualize and quantify regional ventilation changes before and 
      after MCh challenge. We show that difference maps reveal subtle changes in airway 
      caliber, and highlight both focal and diffuse regional alterations in 
      ventilation. Ventilation changes include both hypoventilation and compensatory 
      areas of hyperventilation. The difference maps can be quantified by a histogram 
      plot of the ventilation changes, in which the standard deviation increases with 
      MCh dose (R(2) = 0.89). This method of analysis is shown to be more sensitive 
      than simple threshold-based detection of gross ventilation defects.
CI  - (c) 2010 Wiley-Liss, Inc.
FAU - Mistry, Nilesh N
AU  - Mistry NN
AD  - Department of Biomedical Engineering, Duke University, Durham, North Carolina 
      27710, USA.
FAU - Thomas, Abraham
AU  - Thomas A
FAU - Kaushik, S Sivaram
AU  - Kaushik SS
FAU - Johnson, G Allan
AU  - Johnson GA
FAU - Driehuys, Bastiaan
AU  - Driehuys B
LA  - eng
GR  - P41 RR005959/RR/NCRR NIH HHS/United States
GR  - U24 CA092656/CA/NCI NIH HHS/United States
GR  - U24 CA092656-09/CA/NCI NIH HHS/United States
GR  - R21 HL087094/HL/NHLBI NIH HHS/United States
GR  - P41 RR005959-20/RR/NCRR NIH HHS/United States
GR  - R21 HL087094-02/HL/NHLBI NIH HHS/United States
GR  - R21 HL87094/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Magn Reson Med
JT  - Magnetic resonance in medicine
JID - 8505245
RN  - 0 (Contrast Media)
RN  - 0 (Isotopes)
RN  - 0W5ETF9M2K (Methacholine Chloride)
RN  - 206GF3GB41 (Helium)
SB  - IM
MH  - Administration, Inhalation
MH  - *Algorithms
MH  - Animals
MH  - Asthma/*diagnosis
MH  - Contrast Media/administration & dosage
MH  - *Helium/administration & dosage
MH  - Image Enhancement/*methods
MH  - Isotopes/administration & dosage
MH  - Magnetic Resonance Imaging/*methods
MH  - *Methacholine Chloride
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
PMC - PMC2872792
MID - NIHMS200992
EDAT- 2010/02/27 06:00
MHDA- 2010/06/10 06:00
PMCR- 2011/03/01
CRDT- 2010/02/27 06:00
PHST- 2010/02/27 06:00 [entrez]
PHST- 2010/02/27 06:00 [pubmed]
PHST- 2010/06/10 06:00 [medline]
PHST- 2011/03/01 00:00 [pmc-release]
AID - 10.1002/mrm.22311 [doi]
PST - ppublish
SO  - Magn Reson Med. 2010 Mar;63(3):658-66. doi: 10.1002/mrm.22311.