PMID- 20187449
OWN - NLM
STAT- MEDLINE
DCOM- 20100504
LR  - 20100301
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 24
IP  - 2
DP  - 2010 Feb
TI  - [Transplantation of bone marrow mesenchymal stem cells into spinal cord injury: a 
      comparison of delivery different times].
PG  - 180-4
AB  - OBJECTIVE: To investigate the influence of different transplantation times on the 
      survival and immigration of the bone marrow mesenchymal stem cells (BMSCs) in 
      injured spinal cord by subarachnoid administration, and to evaluate the most 
      optimal subarachnoid administration times for BMSCs. METHODS: Eight adult male 
      rats (weighing 120 g) were used to isolate BMSCs that were cultured, purified and 
      labeled with Hoechst 33342 in vitro. Another 75 adult Wistar rats (weighing 220 
      g) were made the spinal cord injury (SCI) models at T9,10 level according to the 
      improved Allen's method and were randomly divided into 5 groups (groups A, B, C, 
      D, and E, n=15). The labeled BMSCs at 1 x 10(7)/mL 0.1 mL were injected into 
      subarachnoid space of the rats via a catheters under the subarachnoid space in 
      groups A (one time at 1 week), B ( two times at 1 and 3 weeks), C (3 times at 1, 
      3, and 5 weeks) and D (5 times at 1, 3, 5, 7, and 9 weeks) and 0.2 mL 
      phosphate-buffered saline (PBS) was injected in group E (5 times at 1, 3, 5, 7, 
      and 9 weeks) as blank control. The neurological functions were evaluated using 
      the Basso-Beattie-Bresnahan (BBB) scale 1, 3, 5, 7, 9, and 12 weeks after 
      transplantation. The migration, survival, differentiation, and histomorphological 
      changes of BMSCs were observed by HE, immunohistochemistry, and fluorescence 
      microscopy. RESULTS: At 3 weeks after injury, there were significant differences 
      in the BBB scores between group E and groups A, B, C, D (P < 0.01), and between 
      groups A, B and groups C, D (P < 0.01). At 7, 9, and 12 weeks, the BBB scores 
      were significantly higher in groups C and D than in groups A and B (P < 0.01), 
      and in group B than in group A (P < 0.01). There were no significant differences 
      in the BBB scores between groups C and D (P > 0.05). The fluorescence microscopy 
      showed that the transplanted BMSCs survived and grew in the injured region at 3 
      weeks after injury and as time went on, the transplanted cells gradually 
      decreased in group A; in groups B, C, and D, BMSCs count reached the peak values 
      at 5 and 7 weeks and then gradually decreased. At 12 weeks, the survival BMSCs 
      were significantly more in groups C and D than in groups A and B (P < 0.01). HE 
      staining showed that the formation of cavity was observed in each group at 3 
      weeks after injury and the area of cavity gradually decreased in groups A, B, C, 
      and D. At 12 weeks, the area of cavity was the maximal in groups C and D, 
      moderate in groups A and B, and the maximal in group E. The immunohistochemistry 
      staining indicated that the expression of NF-200 was more intense in groups C and 
      D than in groups A and B. The expression of NF-200-positive fibers was more 
      intense in group C. CONCLUSION: Multiple administration of BMSCs promotes the 
      restoration of injured spinal cord and improves neurological functions, and three 
      times for BMSCs transplantation is best.
FAU - Li, Huiying
AU  - Li H
AD  - Department of Spine Surgery, Lanzhou General Hospital, Lanzhou Command of Chinese 
      PLA, Lanzhou Gansu, 730050, P.R. China.
FAU - Wen, Yimin
AU  - Wen Y
FAU - Luo, Yali
AU  - Luo Y
FAU - Lan, Xu
AU  - Lan X
FAU - Wang, Dabin
AU  - Wang D
FAU - Sun, Zhengang
AU  - Sun Z
FAU - Hu, Lingyun
AU  - Hu L
LA  - chi
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
SB  - IM
MH  - Animals
MH  - *Bone Marrow Transplantation/methods
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation/methods
MH  - Rats
MH  - Rats, Wistar
MH  - Spinal Cord Injuries/*surgery
EDAT- 2010/03/02 06:00
MHDA- 2010/05/05 06:00
CRDT- 2010/03/02 06:00
PHST- 2010/03/02 06:00 [entrez]
PHST- 2010/03/02 06:00 [pubmed]
PHST- 2010/05/05 06:00 [medline]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2010 Feb;24(2):180-4.