PMID- 20188214
OWN - NLM
STAT- MEDLINE
DCOM- 20100908
LR  - 20181201
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 10
IP  - 5
DP  - 2010 May
TI  - Inhibitory effect of phytoglycoprotein (24kDa) on allergy-related factors in 
      compound 48/80-induced mast cells in vivo and in vitro.
PG  - 591-9
LID - 10.1016/j.intimp.2010.02.009 [doi]
AB  - Mast cells are involved in immediate allergic reactions such as asthma, allergic 
      rhinitis, and atopic dermatitis. Allergic reactions caused by extracellular 
      allergens such as xenobiotics may become a critical problem in living 
      circumstances. Recently, we isolated and purified glycoprotein from Zanthoxylum 
      piperitum DC fruit (ZPDC), and demonstrated that ZPDC glycoprotein (5-20mg/kg, 
      25-100mug/ml) has an inhibitory effect on allergy-related mediators in the 
      compound 48/80-treated BALB/c and human mast cells (HMC-1 cells). Our results 
      obtained from this study showed that ZPDC glycoprotein (10mg/kg) inhibited 
      interleukin-4 (IL-4), immunoglobulin E (IgE), and histamine are released in mouse 
      serum. Also, ZPDC glycoprotein (50mug/ml) attenuated the degranulation of mast 
      cells, intracellular Ca(2+) levels, and the activities of phosphorylation of p38 
      mitogen-activated protein kinase (MAPK), nuclear factor (NF)-kappaB (p50 and 
      p65), and cyclooxygenase-2 (COX-2) in the HMC-1 cells. Taken together, we 
      speculate that the ZPDC glycoprotein might be one component found in natural 
      products that has the ability to prevent dysfunction in the immune system caused 
      by several different allergens.
CI  - Copyright 2010 Elsevier B.V. All rights reserved.
FAU - Lee, Jin
AU  - Lee J
AD  - Molecular Biochemistry Laboratory, Biotechnology Research Institute, Chonnam 
      National University, 300 Yongbong-Dong, Gwang-ju 500-757, South Korea.
FAU - Lim, Kye-Taek
AU  - Lim KT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100224
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Anti-Allergic Agents)
RN  - 0 (Glycoproteins)
RN  - 0 (Plant Proteins)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 4091-50-3 (p-Methoxy-N-methylphenethylamine)
SB  - IM
MH  - Animals
MH  - Anti-Allergic Agents/*administration & dosage/adverse effects
MH  - Calcium Signaling/drug effects
MH  - Cell Degranulation/drug effects
MH  - Cell Line
MH  - Female
MH  - Fruit
MH  - Glycoproteins/*administration & dosage/adverse effects
MH  - Histamine Release/drug effects
MH  - Humans
MH  - Immunoglobulin E/*biosynthesis/blood/genetics
MH  - Interleukin-4/*biosynthesis/genetics/metabolism
MH  - MAP Kinase Signaling System/drug effects
MH  - Mast Cells/*drug effects/immunology/metabolism/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Plant Proteins/*administration & dosage/adverse effects
MH  - Zanthoxylum
MH  - p-Methoxy-N-methylphenethylamine/administration & dosage
EDAT- 2010/03/02 06:00
MHDA- 2010/09/09 06:00
CRDT- 2010/03/02 06:00
PHST- 2010/01/08 00:00 [received]
PHST- 2010/02/08 00:00 [revised]
PHST- 2010/02/16 00:00 [accepted]
PHST- 2010/03/02 06:00 [entrez]
PHST- 2010/03/02 06:00 [pubmed]
PHST- 2010/09/09 06:00 [medline]
AID - S1567-5769(10)00068-8 [pii]
AID - 10.1016/j.intimp.2010.02.009 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2010 May;10(5):591-9. doi: 10.1016/j.intimp.2010.02.009. 
      Epub 2010 Feb 24.