PMID- 20188535
OWN - NLM
STAT- MEDLINE
DCOM- 20110324
LR  - 20211203
IS  - 1873-4847 (Electronic)
IS  - 0955-2863 (Print)
IS  - 0955-2863 (Linking)
VI  - 22
IP  - 1
DP  - 2011 Jan
TI  - Influence of gestational overfeeding on cardiac morphometry and hypertrophic 
      protein markers in fetal sheep.
PG  - 30-7
LID - 10.1016/j.jnutbio.2009.11.006 [doi]
AB  - Intrauterine overnutrition is associated with development of cardiovascular 
      disease in adulthood although the underlying mechanism has not been precisely 
      elucidated. This study evaluated the effects of maternal overnutrition on fetal 
      cardiac morphometry and hypertrophy-related mRNA/protein expression. Multiparous 
      ewes were fed either 150% of National Research Council (NRC) nutrient 
      requirements (overfed group) or 100% of NRC requirements (control group) from 60 
      days before mating to Day 75 (D75) of gestation, when ewes were euthanized. 
      Cardiac morphometry, histology and expression of Akt, forkhead-3a (Foxo3a), 
      glycogen synthase kinase-3beta (GSK3beta), mammalian target of rapamycin (mTOR), NFATc3 
      and GATA4, atrial natriuretic factor (ANF), calcineurin A and caspase-8 were 
      examined. Crown rump length, left and right ventricular free wall weights and 
      left ventricular wall thickness were increased in D75 overnourished fetuses. 
      Hematoxylin and eosin staining revealed irregular myofiber orientation and 
      increased interstitial space in heart tissues from overfed group. Masson's 
      trichrome staining displayed myofiber hypertrophy and fascicular disarray in 
      heart tissues from overfed group. Overfeeding significantly enhanced Foxo3a 
      phosphorylation in both ventricles, while protein expression of Akt, Foxo3a, 
      GSK3beta and caspase-8 as well as phosphorylated Akt and GSK3beta in either ventricle 
      was unaffected. Overfeeding increased left ventricular mTOR, NFATc3 (both total 
      and phosphorylated) and calcineurin A. GATA4, pGATA4 and ANF expression were 
      unchanged in both ventricles. Collectively, our data suggested that overfeeding 
      during early to mid gestation (D75) leads to morphometric changes without overt 
      pathology which may be related to elevated expression of mTOR, NFATc3, 
      calcineurin A and phosphorylation of Foxo3a, mTOR and NFATc3.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Fan, Xiujuan
AU  - Fan X
AD  - Center for Cardiovascular Research and Alternative Medicine, University of 
      Wyoming, Laramie, WY 82071, USA.
FAU - Turdi, Subat
AU  - Turdi S
FAU - Ford, Stephen P
AU  - Ford SP
FAU - Hua, Yinan
AU  - Hua Y
FAU - Nijland, Mark J
AU  - Nijland MJ
FAU - Zhu, Meijun
AU  - Zhu M
FAU - Nathanielsz, Peter W
AU  - Nathanielsz PW
FAU - Ren, Jun
AU  - Ren J
LA  - eng
GR  - P01 HD021350/HD/NICHD NIH HHS/United States
GR  - P20 RR016474/RR/NCRR NIH HHS/United States
GR  - P20 RR016474-09/RR/NCRR NIH HHS/United States
GR  - P20 RR16474/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20100225
PL  - United States
TA  - J Nutr Biochem
JT  - The Journal of nutritional biochemistry
JID - 9010081
RN  - 0 (Biomarkers)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (NFATC Transcription Factors)
RN  - 0 (Natriuretic Peptides)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 3.1.3.16 (Calcineurin)
SB  - IM
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Calcineurin/metabolism
MH  - Cardiomegaly/*metabolism/*pathology
MH  - Cardiovascular Diseases/epidemiology
MH  - Extracellular Space
MH  - Fascia/pathology
MH  - Female
MH  - Fetal Heart/*metabolism/*pathology
MH  - Forkhead Transcription Factors/metabolism
MH  - Gene Expression Regulation, Developmental
MH  - *Maternal Nutritional Physiological Phenomena
MH  - Myofibrils/pathology
MH  - NFATC Transcription Factors/metabolism
MH  - Natriuretic Peptides/genetics/metabolism
MH  - Overnutrition/*metabolism/*pathology
MH  - Phosphorylation
MH  - RNA, Messenger/metabolism
MH  - Sheep, Domestic
MH  - TOR Serine-Threonine Kinases/metabolism
PMC - PMC2901772
MID - NIHMS184612
EDAT- 2010/03/02 06:00
MHDA- 2011/03/25 06:00
PMCR- 2012/01/01
CRDT- 2010/03/02 06:00
PHST- 2009/08/25 00:00 [received]
PHST- 2009/10/29 00:00 [revised]
PHST- 2009/11/09 00:00 [accepted]
PHST- 2010/03/02 06:00 [entrez]
PHST- 2010/03/02 06:00 [pubmed]
PHST- 2011/03/25 06:00 [medline]
PHST- 2012/01/01 00:00 [pmc-release]
AID - S0955-2863(09)00248-4 [pii]
AID - 10.1016/j.jnutbio.2009.11.006 [doi]
PST - ppublish
SO  - J Nutr Biochem. 2011 Jan;22(1):30-7. doi: 10.1016/j.jnutbio.2009.11.006. Epub 
      2010 Feb 25.