PMID- 20188708 OWN - NLM STAT- MEDLINE DCOM- 20100422 LR - 20181201 IS - 1090-2104 (Electronic) IS - 0006-291X (Linking) VI - 394 IP - 1 DP - 2010 Mar 26 TI - Differential epigenetic regulation of BDNF and NT-3 genes by trichostatin A and 5-aza-2'-deoxycytidine in Neuro-2a cells. PG - 173-7 LID - 10.1016/j.bbrc.2010.02.139 [doi] AB - To understand epigenetic regulation of neurotrophins in Neuro-2a mouse neuroblastoma cells, we investigated the alteration of CpG methylation of brain-derived neurotrophic factor (BDNF) promoter I and neurotrophin-3 (NT-3) promoter IB and that of histone modification in Neuro-2a cells. Bisulfite genomic sequencing showed that the CpG sites of BDNF promoter I were methylated in non-treated Neuro-2a cells and demethylated following 5-aza-2'-deoxycytidine (5-aza-dC) treatment. In contrast, methylation status of the NT-3 promoter IB did not change by 5-aza-dC treatment in Neuro-2a cells. Furthermore, we demonstrated that BDNF exon I-IX mRNA was induced by trichostatin A (TSA) treatment. However, NT-3 exon IB-II mRNA was not induced by TSA treatment. Chromatin immunoprecipitation assays showed that the levels of acetylated histones H3 and H4 on BDNF promoter I were increased by TSA. These results demonstrate that DNA methylation and/or histone modification regulate BDNF gene expression, but do not regulate NT-3 gene expression in Neuro-2a cells. CI - Copyright (c) 2010 Elsevier Inc. All rights reserved. FAU - Ishimaru, Naoki AU - Ishimaru N AD - Faculty of Pharmaceutical Sciences, International University of Health and Welfare, 2600-1 Ootawara, Tochigi 324-8501, Japan. FAU - Fukuchi, Mamoru AU - Fukuchi M FAU - Hirai, Akina AU - Hirai A FAU - Chiba, Yusuke AU - Chiba Y FAU - Tamura, Tomonari AU - Tamura T FAU - Takahashi, Nami AU - Takahashi N FAU - Tabuchi, Akiko AU - Tabuchi A FAU - Tsuda, Masaaki AU - Tsuda M FAU - Shiraishi, Masahiko AU - Shiraishi M LA - eng PT - Journal Article DEP - 20100225 PL - United States TA - Biochem Biophys Res Commun JT - Biochemical and biophysical research communications JID - 0372516 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Histones) RN - 0 (Hydroxamic Acids) RN - 0 (Nerve Growth Factors) RN - 0 (neurotropin 3, mouse) RN - 3X2S926L3Z (trichostatin A) RN - 776B62CQ27 (Decitabine) RN - EC 2.1.1.- (DNA Modification Methylases) RN - M801H13NRU (Azacitidine) SB - IM MH - Acetylation MH - Animals MH - Azacitidine/analogs & derivatives/pharmacology MH - Brain-Derived Neurotrophic Factor/*genetics MH - Cell Line, Tumor MH - Chromatin Immunoprecipitation MH - CpG Islands MH - *DNA Methylation MH - DNA Modification Methylases/antagonists & inhibitors MH - Decitabine MH - *Epigenesis, Genetic MH - Exons/genetics MH - Histones/metabolism MH - Hydroxamic Acids/pharmacology MH - Mice MH - Nerve Growth Factors/*genetics MH - Neurons/drug effects/*metabolism MH - Promoter Regions, Genetic EDAT- 2010/03/02 06:00 MHDA- 2010/04/23 06:00 CRDT- 2010/03/02 06:00 PHST- 2010/02/16 00:00 [received] PHST- 2010/02/22 00:00 [accepted] PHST- 2010/03/02 06:00 [entrez] PHST- 2010/03/02 06:00 [pubmed] PHST- 2010/04/23 06:00 [medline] AID - S0006-291X(10)00373-6 [pii] AID - 10.1016/j.bbrc.2010.02.139 [doi] PST - ppublish SO - Biochem Biophys Res Commun. 2010 Mar 26;394(1):173-7. doi: 10.1016/j.bbrc.2010.02.139. Epub 2010 Feb 25.