PMID- 20188888
OWN - NLM
STAT- MEDLINE
DCOM- 20100520
LR  - 20131121
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 81
IP  - 1-2
DP  - 2010 Apr 15
TI  - Aptamer-Au NPs conjugates-accumulated methylene blue for the sensitive 
      electrochemical immunoassay of protein.
PG  - 63-7
LID - 10.1016/j.talanta.2009.11.035 [doi]
AB  - In this paper, we combine the advantages of aptamer, nanomaterial and antibody to 
      design an electrochemical sandwich immunoassay for the ultrasensitive detection 
      of human immunoglobulin E (IgE) by using methylene blue (MB) as electrochemical 
      indicator. The sandwich structure is fabricated by using goat anti-human IgE as 
      capturing probe. Aptamer-Au nanoparticles (NPs) conjugates are used both as a 
      sandwich amplification element as well as an accumulation reagent of MB. Once the 
      aptamer-Au NPs conjugates specifically bind to electrode surface, MB molecules 
      are accumulated on its surface by the specific interaction of MB with G base of 
      aptamer-Au NPs conjugates. Therefore, with the increase of human IgE 
      concentration, more aptamer-Au-NPs conjugates are bound, and thus, more MB 
      molecules are accumulated. A good linear relationship is obtained for the 
      detection of human IgE over a range of 1-10,000 ng/ml with a lowest detection 
      limit of 0.52 ng/ml. In addition, by using BSA, human IgA and human IgM as 
      contrast, the excellent specificity of this sensing system for the detection of 
      human IgE is also demonstrated.
CI  - (c) 2009 Elsevier B.V. All rights reserved.
FAU - Wang, Jianlong
AU  - Wang J
AD  - Department of Chemical Engineering, Worcester Polytechnic Institute, 100 
      Institute Road, Worcester, MA 01609, USA.
FAU - Munir, Ahsan
AU  - Munir A
FAU - Li, Zhonghong
AU  - Li Z
FAU - Zhou, H Susan
AU  - Zhou HS
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20091117
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Antibodies)
RN  - 0 (Aptamers, Nucleotide)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 7440-57-5 (Gold)
RN  - T42P99266K (Methylene Blue)
SB  - IM
MH  - Animals
MH  - Antibodies/immunology
MH  - Aptamers, Nucleotide/*chemistry/metabolism
MH  - Binding Sites
MH  - Biosensing Techniques/*methods
MH  - Cattle
MH  - Electrochemistry
MH  - Gold/*chemistry
MH  - Humans
MH  - Immunoassay/*methods
MH  - Immunoglobulin E/*analysis/immunology/metabolism
MH  - Limit of Detection
MH  - Metal Nanoparticles/*chemistry
MH  - Methylene Blue/*chemistry
EDAT- 2010/03/02 06:00
MHDA- 2010/05/21 06:00
CRDT- 2010/03/02 06:00
PHST- 2009/08/20 00:00 [received]
PHST- 2009/11/10 00:00 [revised]
PHST- 2009/11/11 00:00 [accepted]
PHST- 2010/03/02 06:00 [entrez]
PHST- 2010/03/02 06:00 [pubmed]
PHST- 2010/05/21 06:00 [medline]
AID - S0039-9140(09)00884-4 [pii]
AID - 10.1016/j.talanta.2009.11.035 [doi]
PST - ppublish
SO  - Talanta. 2010 Apr 15;81(1-2):63-7. doi: 10.1016/j.talanta.2009.11.035. Epub 2009 
      Nov 17.