PMID- 20188903 OWN - NLM STAT- MEDLINE DCOM- 20100520 LR - 20171116 IS - 1873-3573 (Electronic) IS - 0039-9140 (Linking) VI - 81 IP - 1-2 DP - 2010 Apr 15 TI - Preparation and characteristics of protein molecularly imprinted membranes on the surface of multiwalled carbon nanotubes. PG - 162-6 LID - 10.1016/j.talanta.2009.11.052 [doi] AB - A novel protein molecularly imprinted membrane (PMIM) was synthesized on the surface of multiwalled carbon nanotubes (MWNTs) through a surface molecular imprinting technique by using bovine serum albumin (BSA) as the template molecule, acrylamide (AAm) as the functional monomer, N,N'-methylenebisacrylamide (NNMBA) as the cross-linker and ammonium persulphate [(NH(4))(2)S(2)O(8)] as the initiator. The amounts of raw materials were optimized in this paper and the suitable amount is 0.1g of CNTs, 0.02 g of BSA, 0.24 g of AAm, 0.1g of MBA and 0.025 mg of (NH(4))(2)S(2)O(8). The selective recognition ability of PMIM/MWNTs was evaluated using adsorption experiments. Maximum adsorption capacity was 5.53 microg/mg PMIM/MWNTs and a saturation value was achieved at a BSA concentration of 0.2mg/mL. The selectivity adsorption experiments showed that the PMIM/MWNTs also had higher adsorption capacities for BSA than for such molecules, as HSA, HB, pepsin and HRP. The PMIM/MWNTs displayed a 2.6-fold increase in affinity to BSA compared to the nPMIM/MWNTs. The PMIM/MWNTs, on the other hand, did not exhibit any significant change in affinity to other molecules compared to the nPMIM/MWNTs. In the experiment, the characteristics of the PMIM/MWNTs were analyzed using infrared spectroscopy and their configurations were observed using scanning electron microscopy. CI - (c) 2009 Elsevier B.V. All rights reserved. FAU - Zhang, Maosheng AU - Zhang M AD - Department of Chemistry and Environmental Science, Fujian Province University Key Laboratory of Analytical Science, Zhangzhou Normal University, Zhangzhou 363000, China. FAU - Huang, Jiarong AU - Huang J FAU - Yu, Ping AU - Yu P FAU - Chen, Xi AU - Chen X LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20091202 PL - Netherlands TA - Talanta JT - Talanta JID - 2984816R RN - 0 (Acrylamides) RN - 0 (Cross-Linking Reagents) RN - 0 (Membranes, Artificial) RN - 0 (Nanotubes, Carbon) RN - 20R035KLCI (Acrylamide) RN - 22QF6L357F (ammonium peroxydisulfate) RN - 27432CM55Q (Serum Albumin, Bovine) RN - EDK4RIE19C (N,N'-methylenebisacrylamide) RN - SU46BAM238 (Ammonium Sulfate) SB - IM MH - Acrylamide/chemistry MH - Acrylamides/chemistry MH - Adsorption MH - Ammonium Sulfate/chemistry MH - Animals MH - Cattle MH - Cross-Linking Reagents/chemistry MH - Kinetics MH - *Membranes, Artificial MH - Microscopy, Electron, Scanning MH - Molecular Imprinting/*methods MH - Nanotubes, Carbon/*chemistry MH - Serum Albumin, Bovine/*chemistry MH - Spectroscopy, Fourier Transform Infrared MH - Substrate Specificity MH - Surface Properties EDAT- 2010/03/02 06:00 MHDA- 2010/05/21 06:00 CRDT- 2010/03/02 06:00 PHST- 2009/08/20 00:00 [received] PHST- 2009/11/19 00:00 [revised] PHST- 2009/11/23 00:00 [accepted] PHST- 2010/03/02 06:00 [entrez] PHST- 2010/03/02 06:00 [pubmed] PHST- 2010/05/21 06:00 [medline] AID - S0039-9140(09)00901-1 [pii] AID - 10.1016/j.talanta.2009.11.052 [doi] PST - ppublish SO - Talanta. 2010 Apr 15;81(1-2):162-6. doi: 10.1016/j.talanta.2009.11.052. Epub 2009 Dec 2.