PMID- 20189733 OWN - NLM STAT- MEDLINE DCOM- 20101221 LR - 20100816 IS - 1873-2542 (Electronic) IS - 0378-1135 (Linking) VI - 144 IP - 3-4 DP - 2010 Aug 26 TI - Lethal toxin of Clostridium sordellii is associated with fatal equine atypical myopathy. PG - 487-92 LID - 10.1016/j.vetmic.2010.01.024 [doi] AB - The lethal toxin of Clostridium sordellii (TcsL) evokes severe, mostly fatal disease patterns like toxic shock syndrome in humans and animals. Since this large clostridial toxin-induced severe muscle damaging when injected intramuscularly into mice, we hypothesized that TcsL is also associated with equine atypical myopathy (EAM), a fatal myodystrophy of hitherto unknown etiology. Transmission electron microscopy revealed skeletal and heart muscles of EAM-affected horses to undergo degeneration ultrastructurally similar to the damage found in TcsL-treated mice. Performing immunohistochemistry, myofibers of EAM-affected horses specifically reacted with sera derived from horses with EAM as well as an antibody specific for the N-terminal part of TcsL, while both antibodies failed to bind to the myofibers of either healthy horses or those with other myopathies. The presence of TcsL in myofibers of horses with EAM suggests that it plays a role as trigger or even as lethal factor in this disease. CI - Copyright 2010 Elsevier B.V. All rights reserved. FAU - Unger-Torroledo, Lucia AU - Unger-Torroledo L AD - Equine Clinic, Department of Veterinary Clinical Studies, Vetsuisse Faculty, University of Bern, Bern, Switzerland. FAU - Straub, Reto AU - Straub R FAU - Lehmann, Andrea D AU - Lehmann AD FAU - Graber, Franziska AU - Graber F FAU - Stahl, Christina AU - Stahl C FAU - Frey, Joachim AU - Frey J FAU - Gerber, Vinzenz AU - Gerber V FAU - Hoppeler, Hans AU - Hoppeler H FAU - Baum, Oliver AU - Baum O LA - eng PT - Journal Article DEP - 20100201 PL - Netherlands TA - Vet Microbiol JT - Veterinary microbiology JID - 7705469 RN - 0 (Bacterial Toxins) RN - 0 (lethal toxin LT, Clostridium sordellii) SB - IM MH - Animals MH - Bacterial Toxins/*isolation & purification/metabolism MH - Clostridium Infections/microbiology/*veterinary MH - Clostridium sordellii/*metabolism MH - Female MH - Horse Diseases/*microbiology MH - Horses MH - Male MH - Muscle, Skeletal/pathology/ultrastructure MH - Muscular Diseases/*microbiology MH - Myocardium/ultrastructure EDAT- 2010/03/02 06:00 MHDA- 2010/12/22 06:00 CRDT- 2010/03/02 06:00 PHST- 2010/01/20 00:00 [received] PHST- 2010/01/25 00:00 [accepted] PHST- 2010/03/02 06:00 [entrez] PHST- 2010/03/02 06:00 [pubmed] PHST- 2010/12/22 06:00 [medline] AID - S0378-1135(10)00060-X [pii] AID - 10.1016/j.vetmic.2010.01.024 [doi] PST - ppublish SO - Vet Microbiol. 2010 Aug 26;144(3-4):487-92. doi: 10.1016/j.vetmic.2010.01.024. Epub 2010 Feb 1.