PMID- 20190394
OWN - NLM
STAT- MEDLINE
DCOM- 20100920
LR  - 20190720
IS  - 1347-5215 (Electronic)
IS  - 0918-6158 (Linking)
VI  - 33
IP  - 3
DP  - 2010
TI  - Determination of dehydroascorbic acid in mouse tissues and plasma by using 
      tris(2-carboxyethyl)phosphine hydrochloride as reductant in metaphosphoric 
      acid/ethylenediaminetetraacetic acid solution.
PG  - 364-9
AB  - Ascorbic acid (AA) has a strong anti-oxidant function evident as its ability to 
      scavenge superoxide radicals in vitro. Moreover, AA is an essential ingredient 
      for post-translational proline hydroxylation of collagen molecules. 
      Dehydroascorbic acid (DHA), the oxidized form of AA, is generated from these 
      reactions. In this study, we describe an improved method for assessing DHA in 
      biological samples. The use of 35 mM tris(2-carboxyethyl)phosphine hydrochloride 
      (TCEP) as a reductant completely reduced DHA to AA after 2 h on ice in a 5% 
      solution of metaphosphoric acid containing 1 mM ethylenediaminetetraacetic acid 
      (EDTA) at pH 1.5. This method enabled us to measure the DHA content in multiple 
      tissues and plasma of 6-weeks-old mice. The percentages of DHA per total AA 
      differed markedly among these tissues, i.e., from 0.8 to 19.5%. The lung, heart, 
      spleen and plasma had the highest levels at more than 10% of DHA per total AA 
      content, whereas the cerebrum, cerebellum, liver, kidney and small intestine had 
      less than 5% of DHA per total AA content. This difference in DHA content may 
      indicate an important disparity of oxidative stress levels among physiologic 
      sites. Therefore, this improved method provides a useful standard for all DHA 
      determinations.
FAU - Sato, Yasunori
AU  - Sato Y
AD  - Department of Biochemistry, Faculty of Pharmaceutical Sciences, Toho University, 
      Chiba 274-8510, Japan.
FAU - Uchiki, Takayuki
AU  - Uchiki T
FAU - Iwama, Mizuki
AU  - Iwama M
FAU - Kishimoto, Yuki
AU  - Kishimoto Y
FAU - Takahashi, Ryoya
AU  - Takahashi R
FAU - Ishigami, Akihito
AU  - Ishigami A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Antioxidants)
RN  - 0 (Phosphines)
RN  - 0 (Phosphorous Acids)
RN  - 0 (Reducing Agents)
RN  - 22OAC2MO2S (tris(2-carboxyethyl)phosphine)
RN  - 9G34HU7RV0 (Edetic Acid)
RN  - MTK99R3UV0 (metaphosphoric acid)
RN  - PQ6CK8PD0R (Ascorbic Acid)
RN  - Y2Z3ZTP9UM (Dehydroascorbic Acid)
SB  - IM
MH  - Animals
MH  - Antioxidants/*analysis/metabolism
MH  - Ascorbic Acid/*analysis/metabolism
MH  - Clinical Laboratory Techniques/*methods
MH  - Dehydroascorbic Acid/*analysis/blood
MH  - Edetic Acid/pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Organ Specificity
MH  - Oxidative Stress
MH  - Phosphines/*analysis
MH  - Phosphorous Acids/pharmacology
MH  - Reducing Agents/pharmacology
EDAT- 2010/03/02 06:00
MHDA- 2010/09/21 06:00
CRDT- 2010/03/02 06:00
PHST- 2010/03/02 06:00 [entrez]
PHST- 2010/03/02 06:00 [pubmed]
PHST- 2010/09/21 06:00 [medline]
AID - JST.JSTAGE/bpb/33.364 [pii]
AID - 10.1248/bpb.33.364 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2010;33(3):364-9. doi: 10.1248/bpb.33.364.