PMID- 20190624
OWN - NLM
STAT- MEDLINE
DCOM- 20100525
LR  - 20121115
IS  - 1528-1159 (Electronic)
IS  - 0362-2436 (Linking)
VI  - 35
IP  - 5
DP  - 2010 Mar 1
TI  - Targeted retrograde gene delivery of brain-derived neurotrophic factor suppresses 
      apoptosis of neurons and oligodendroglia after spinal cord injury in rats.
PG  - 497-504
LID - 10.1097/BRS.0b013e3181b8e89b [doi]
AB  - STUDY DESIGN: Histologic and immunohistochemical studies after targeted 
      retrograde adenovirus (AdV)-mediated brain-derived neurotrophic factor (BDNF) 
      gene delivery via intramuscular injection in rats with injured spinal cord. 
      OBJECTIVE: To investigate the neuroprotective effect of targeted retrograde 
      AdV-BDNF gene transfection in the traumatically injured spinal cord in terms of 
      prevention of apoptosis of neurons and oligodendrocytes. SUMMARY OF BACKGROUND 
      DATA: Several studies investigated the neuroprotective effects of neurotrophins 
      including BDNF on spinal cord injury, with respect to prevention of neural cell 
      apoptosis in injured spinal cord. However, no report has described the potential 
      effect of targeted retrograde neurotrophic factor gene delivery in injured spinal 
      cord on prevention of neural cell apoptosis. METHODS: AdV-BDNF or AdV-LacZ was 
      used for retrograde delivery via bilateral sternomastoid muscles to the spinal 
      accessory motoneurons immediately after spinal cord injury in rats. Localization 
      of beta-galactosidase expression produced by LacZ gene or AdV-BDNF gene 
      transfection was examined by immunofluorescence staining and double staining of 
      cell markers (NeuN, RIP, GFAP, OX-42, and NG2) in the injured spinal cord. 
      TUNEL-positive cells were counted and immunoreactivity to active caspase-3 and 
      NG2 was examined after gene injection. RESULTS: Retrograde delivery of LacZ 
      marker gene was identified in cervical spinal neurons and glial cells including 
      oligodendrocytes in the white matter.AdV-BDNF transfection resulted in a 
      significant decrease in the number of TUNEL-positive apoptotic cells by 
      downregulating the caspase apoptotic pathway, with significant promotion of NG2 
      expression in injured spinal cord, compared with AdV-LacZ injection. CONCLUSION: 
      Our results suggest that targeted retrograde BDNF gene delivery suppresses 
      apoptosis of neurons and oligodendrocytes in the injured rat spinal cord.
FAU - Nakajima, Hideaki
AU  - Nakajima H
AD  - Department of Orthopaedics and Rehabilitation Medicine, The University of Fukui, 
      Fukui, Japan.
FAU - Uchida, Kenzo
AU  - Uchida K
FAU - Yayama, Takafumi
AU  - Yayama T
FAU - Kobayashi, Shigeru
AU  - Kobayashi S
FAU - Guerrero, Alexander Rodriguez
AU  - Guerrero AR
FAU - Furukawa, Shoei
AU  - Furukawa S
FAU - Baba, Hisatoshi
AU  - Baba H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Spine (Phila Pa 1976)
JT  - Spine
JID - 7610646
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Adenoviridae/genetics
MH  - Animals
MH  - Apoptosis/*genetics
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/*genetics/metabolism
MH  - Cervical Vertebrae
MH  - Genetic Therapy
MH  - Genetic Vectors/genetics
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Neurons/*metabolism/pathology
MH  - Oligodendroglia/*metabolism/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord Injuries/*genetics/pathology/*therapy
MH  - Statistics, Nonparametric
EDAT- 2010/03/02 06:00
MHDA- 2010/05/26 06:00
CRDT- 2010/03/02 06:00
PHST- 2010/03/02 06:00 [entrez]
PHST- 2010/03/02 06:00 [pubmed]
PHST- 2010/05/26 06:00 [medline]
AID - 00007632-201003010-00006 [pii]
AID - 10.1097/BRS.0b013e3181b8e89b [doi]
PST - ppublish
SO  - Spine (Phila Pa 1976). 2010 Mar 1;35(5):497-504. doi: 
      10.1097/BRS.0b013e3181b8e89b.