PMID- 20193234
OWN - NLM
STAT- MEDLINE
DCOM- 20100528
LR  - 20221207
IS  - 0254-6450 (Print)
IS  - 0254-6450 (Linking)
VI  - 30
IP  - 9
DP  - 2009 Sep
TI  - [Amplification and clinical significance of hTERC gene in the cervical exfoliated 
      cells from natural population in Shenzhen].
PG  - 946-50
AB  - OBJECTIVE: To study the amplification of human telomerase RNA component (hTERC) 
      gene in the cervical exfoliated cells from natural population in Shenzhen and to 
      explore its relationship with human pappiloma-virus (HPV) infection, abnormal 
      cervical cytology and cervical intraepithelial neoplasia (CIN). METHODS: Three 
      hundred and eighty-eight women, 30 - 59 year-old and having normal intelligence 
      in a community of Shenzhen, were examined with liquid-based cytology. Human 
      pappiloma-virus (HR-HPV) was tested by hybrid capture II (HC-II) and hTERC gene 
      detection by fluorescence in situ hybridization (FISH). Patients with ASCUS and 
      above lesion, and/or positive HR-HPV results and/or amplification of hTERC gene 
      were examined by colposcopy, multiple biopsies of cervical quadrant and 
      pathology. RESULTS: The frequencies of CIN I, II, III, cervical cancer were 55 
      (14.18%), 4 (1.03%), 9 (2.32%) and 1 (0.26%) respectively, with the rate of hTERC 
      gene amplification as 8.76%. There were significantly differences of hTERC 
      amplification among the samples with different cytological and histological 
      lesions as well with HPV infection (P < 0.01). (1) The positive rate of HPV 
      infection was 17.01%; the positive rates of hTERC gene amplification were 19.70% 
      in HPV positive and 6.52% in HPV negative samples and the results were 
      significantly different (P < 0.01). (2) Cytologically, the rates of hTERC gene 
      amplification appeared to be as follows: NILM (5.97%), ASCUS (18.75%), LSIL 
      (10.00%), ASC-H (66.67%), HSIL (100.00%). There was a marked increase of hTERC 
      amplification in patients with HSIL and above lesions (P < 0.01). (3) On 
      histology findings, the rates of hTERC gene amplification were as follows: NILM 
      (0%), CIN I (5.45%), CIN II (50.00%), CIN III (77.78%), and invasive carcinoma 
      (100.00%). There was a marked increase of hTERC amplification in patients with 
      CIN II and above lesions (P < 0.01). CONCLUSION: There was a close correlation 
      between amplification of hTERC and histological as well cytological lesions which 
      increased progressively along with the severity of cytological and histological 
      grade. The evidence of hTERC, with or without amplification, might serve as a 
      prognostic indicator to measure the grade of lesion.
FAU - Li, Yi-bing
AU  - Li YB
AD  - Department of Obstetrics and Gynecology of Peking University Shenzhen Hospital, 
      Shenzhen 518036, China.
FAU - Wulan, Na
AU  - Wulan N
FAU - Liu, Zhi-hong
AU  - Liu ZH
FAU - Li, Juan
AU  - Li J
FAU - Wang, Chun
AU  - Wang C
FAU - Zhou, Yan-qiu
AU  - Zhou YQ
FAU - Wu, Rui-fang
AU  - Wu RF
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Liu Xing Bing Xue Za Zhi
JT  - Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi
JID - 8208604
RN  - 0 (telomerase RNA)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Adult
MH  - Cervix Uteri/*pathology
MH  - China
MH  - Colposcopy
MH  - Female
MH  - *Gene Amplification
MH  - Genetic Predisposition to Disease
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Papillomavirus Infections/*genetics/pathology
MH  - Prognosis
MH  - RNA/*genetics
MH  - Telomerase/*genetics
MH  - Uterine Cervical Dysplasia/*genetics/pathology
EDAT- 2010/03/03 06:00
MHDA- 2010/05/29 06:00
CRDT- 2010/03/03 06:00
PHST- 2010/03/03 06:00 [entrez]
PHST- 2010/03/03 06:00 [pubmed]
PHST- 2010/05/29 06:00 [medline]
PST - ppublish
SO  - Zhonghua Liu Xing Bing Xue Za Zhi. 2009 Sep;30(9):946-50.