PMID- 20195281
OWN - NLM
STAT- MEDLINE
DCOM- 20110308
LR  - 20180822
IS  - 1440-1711 (Electronic)
IS  - 0818-9641 (Linking)
VI  - 88
IP  - 6
DP  - 2010 Aug
TI  - Cross-presentation of HCMV chimeric protein enables generation and measurement of 
      polyclonal T cells.
PG  - 676-84
LID - 10.1038/icb.2010.20 [doi]
AB  - CD8(+) T cell immunity has a critical function in controlling human 
      cytomegalovirus (HCMV) infection. In immunocompromized individuals, HCMV 
      reactivation or disease can lead to increased morbidity and mortality, 
      particularly in transplant recipients. In this setting, adoptive transfer of 
      HCMV-specific CD8(+) T cells is a promising vaccine strategy to restore viral 
      immunity, with most clinical approaches focussing on the use of peptides for the 
      generation of single epitope-specific CD8(+) T cells. We show that using an 
      IE1-pp65 chimeric protein as the antigen source promotes effective 
      cross-presentation, by monocyte-derived dendritic cells (MoDCs), to generate 
      polyclonal CD8(+) T cell epitopes. By exploring human leukocyte antigen 
      (HLA)-restricted immunodominance hierarchies both within and across two 
      immunodominant proteins, we show that HLA-B7 epitopes elicit higher CD8(+) T cell 
      responses compared with HLA-A1, -A2 or -B8. This study provides important 
      evidence highlighting both the efficacy of the IE1-pp65 chimeric protein and the 
      importance of immunodominance in designing future therapeutic vaccines.
FAU - Nguyen, Thi H O
AU  - Nguyen TH
AD  - Malignant Haematology and Stem Cell Transplantation Service, The Alfred Hospital, 
      Victoria, Australia.
FAU - Sullivan, Lucy C
AU  - Sullivan LC
FAU - Kotsimbos, Tom C
AU  - Kotsimbos TC
FAU - Schwarer, Anthony P
AU  - Schwarer AP
FAU - Mifsud, Nicole A
AU  - Mifsud NA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100302
PL  - United States
TA  - Immunol Cell Biol
JT  - Immunology and cell biology
JID - 8706300
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA Antigens)
RN  - 0 (IE1-pp65 recombinant protein, Human cytomegalovirus)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Immunodominant Epitopes)
RN  - 0 (Phosphoproteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Matrix Proteins)
SB  - IM
MH  - CD8-Positive T-Lymphocytes/immunology/*metabolism/pathology
MH  - Cross-Priming
MH  - Cytomegalovirus/*immunology/pathogenicity
MH  - Cytomegalovirus Infections/*immunology/therapy/virology
MH  - Dendritic Cells/immunology/*metabolism/pathology
MH  - Epitopes, T-Lymphocyte/genetics/*metabolism
MH  - HLA Antigens/immunology/metabolism
MH  - Humans
MH  - Immediate-Early Proteins/genetics/immunology/*metabolism
MH  - Immunodominant Epitopes/genetics/*metabolism
MH  - *Immunotherapy, Adoptive
MH  - Lymphocyte Activation
MH  - Monocytes/pathology
MH  - Phosphoproteins/genetics/immunology/*metabolism
MH  - Protein Binding/immunology
MH  - Recombinant Proteins/genetics/immunology/metabolism
MH  - Viral Matrix Proteins/genetics/immunology/*metabolism
MH  - Virus Activation
EDAT- 2010/03/03 06:00
MHDA- 2011/03/09 06:00
CRDT- 2010/03/03 06:00
PHST- 2010/03/03 06:00 [entrez]
PHST- 2010/03/03 06:00 [pubmed]
PHST- 2011/03/09 06:00 [medline]
AID - icb201020 [pii]
AID - 10.1038/icb.2010.20 [doi]
PST - ppublish
SO  - Immunol Cell Biol. 2010 Aug;88(6):676-84. doi: 10.1038/icb.2010.20. Epub 2010 Mar 
      2.