PMID- 20196105
OWN - NLM
STAT- MEDLINE
DCOM- 20110203
LR  - 20121115
IS  - 1097-0029 (Electronic)
IS  - 1059-910X (Linking)
VI  - 73
IP  - 11
DP  - 2010 Oct
TI  - Low-temperature glycol methacrylate resin embedding method: A protocol suitable 
      for bone marrow immunohistochemistry, PCR, and fish analysis.
PG  - 1067-71
LID - 10.1002/jemt.20836 [doi]
AB  - Molecular analyses such as fluorescence in situ hybridization (FISH) and 
      polymerase chain reaction (PCR) are demanded to improve diagnostic accuracy in 
      addition to immunohistopathology of bone marrow (BM) trephine specimens. 
      Conventional BM embedding method needs decalcification, and its procedure may 
      impair tissue morphology and DNA quality. Here, we report an undecalcified method 
      by which glycol methacrylate resin is polymerized at low temperature (4 degrees C). Using 
      this method, BM enzyme activity and antigenic determinants are well preserved, 
      and moreover, DNA extracted from plastic embedding sections is suitable for PCR 
      amplification and sequencing, FISH analysis can be well done because of the DNA 
      integrity of BM sections. If working with BM trephine specimen, our protocol 
      offers the possibility to combine superior morphology with modern molecular 
      analysis.
CI  - (c) 2010 Wiley-Liss, Inc.
FAU - Zhang, Qiguo
AU  - Zhang Q
AD  - Department of Hematology, The Affiliated Drum Tower Hospital of Nanjing 
      University Medical School, Nanjing 210008, China.
FAU - Wang, Jing
AU  - Wang J
FAU - Wu, Hongyan
AU  - Wu H
FAU - Zhang, Le
AU  - Zhang L
FAU - Zhou, Jinyong
AU  - Zhou J
FAU - Ye, Qing
AU  - Ye Q
FAU - Shao, Xiaoyan
AU  - Shao X
FAU - Guan, Chaoyang
AU  - Guan C
FAU - Xu, Jingyan
AU  - Xu J
FAU - Yang, Yonggong
AU  - Yang Y
FAU - Zhou, Rongfu
AU  - Zhou R
FAU - Ouyang, Jian
AU  - Ouyang J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Microsc Res Tech
JT  - Microscopy research and technique
JID - 9203012
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, CD34)
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Methacrylates)
RN  - 6E1I4IV47V (hydroxyethyl methacrylate)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.10.2 (JAK2 protein, human)
RN  - EC 2.7.10.2 (Janus Kinase 2)
SB  - IM
MH  - Antibodies, Monoclonal/metabolism
MH  - Antigens, CD34/chemistry/metabolism
MH  - Bone Marrow/*chemistry/metabolism/ultrastructure
MH  - DNA/chemistry/isolation & purification
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/chemistry/metabolism
MH  - Immunohistochemistry/*methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Janus Kinase 2/chemistry/metabolism
MH  - Leukemia
MH  - Methacrylates/*chemistry
MH  - Polymerase Chain Reaction/*methods
MH  - Polymorphism, Restriction Fragment Length
MH  - Tissue Embedding/*methods
EDAT- 2010/03/03 06:00
MHDA- 2011/02/04 06:00
CRDT- 2010/03/03 06:00
PHST- 2010/03/03 06:00 [entrez]
PHST- 2010/03/03 06:00 [pubmed]
PHST- 2011/02/04 06:00 [medline]
AID - 10.1002/jemt.20836 [doi]
PST - ppublish
SO  - Microsc Res Tech. 2010 Oct;73(11):1067-71. doi: 10.1002/jemt.20836.