PMID- 20198306
OWN - NLM
STAT- MEDLINE
DCOM- 20100519
LR  - 20100303
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 25
IP  - 4
DP  - 2010 Apr
TI  - Expression of MLN64 influences cellular matrix adhesion of breast cancer cells, 
      the role for focal adhesion kinase.
PG  - 573-80
AB  - The metastatic lymph node 64 (MLN64) gene was initially identified as highly 
      expressed in the metastatic lymph node from breast cancer. It is localized in 
      q12-q21 of the human chromosome 17 and is often co-amplified with erbB-2. 
      However, the role played by MLN64 in breast cancer remains unclear. In the 
      present study, the expression of MLN64 was examined in a breast cancer cohort 
      using quantitative real-time PCR and immunohistochemical staining. It 
      demonstrated that MLN64 was highly expressed in breast tumours compared to 
      corresponding background tissues at both transcript level and protein level. The 
      elevated level of MLN64 transcripts was correlated with the poor prognosis and 
      overall survival of the patients. A panel of breast cancer cell sublines was 
      subsequently developed by knockdown of MLN64 expression. Loss of MLN64 expression 
      in MCF-7 cells resulted in a significant reduction of cell growth (absorbance for 
      MCF-7DeltaMLN64 being 0.87+/-0.07, P<0.01 vs. wild-type control (MCF-7WT 
      1.13+/-0.06) and transfection control (MCF-7pEF 1.27+/-0.05). In cell-matrix 
      adhesion assay, MDA-MB-231DeltaMLN64 cells showed a significant increase in 
      adhesion (86+/-14), p<0.01 compared with both MDA-MB-231WT (61+/-20) and 
      MDA-MB-231pEF (45+/-27). Further investigations demonstrated an increase in 
      protein level of the focal adhesion kinase (FAK) in MDA-MB-231DeltaMLN64 cells 
      using Western blot analysis and immunofluorescent staining of FAK. Moreover, 
      addition of FAK inhibitor to these cells diminished the effect of MLN64 on 
      cell-matrix adhesion, suggesting that FAK contributed to the increased adhesion 
      in MDA-MB-231DeltaMLN64 cells. In conclusion, MLN64 is overexpressed in breast 
      cancer, and its level correlates with poor prognosis and patient survival. MLN64 
      contributes to the development and progression of breast cancer through the 
      regulation of cell proliferation and adhesive capacity.
FAU - Cai, Wei
AU  - Cai W
AD  - Metastasis and Angiogenesis Research Group, Department of Surgery, Cardiff 
      University School of Medicine, Heath Park, Cardiff CF14 4XN, UK.
FAU - Ye, Lin
AU  - Ye L
FAU - Sun, Jiabang
AU  - Sun J
FAU - Mansel, Robert E
AU  - Mansel RE
FAU - Jiang, Wen G
AU  - Jiang WG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Carrier Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 0 (STARD3 protein, human)
RN  - EC 2.7.10.2 (Focal Adhesion Protein-Tyrosine Kinases)
SB  - IM
MH  - Breast Neoplasms/*enzymology/genetics/*pathology
MH  - Carrier Proteins/genetics/*metabolism
MH  - Cell Adhesion/drug effects
MH  - Cell Line, Tumor
MH  - Extracellular Matrix/drug effects/*metabolism
MH  - Female
MH  - Focal Adhesion Protein-Tyrosine Kinases/antagonists & inhibitors/*metabolism
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Membrane Proteins/genetics/*metabolism
MH  - Protein Kinase Inhibitors/pharmacology
MH  - RNA, Messenger/genetics/metabolism
MH  - Survival Analysis
MH  - Treatment Outcome
EDAT- 2010/03/04 06:00
MHDA- 2010/05/21 06:00
CRDT- 2010/03/04 06:00
PHST- 2010/03/04 06:00 [entrez]
PHST- 2010/03/04 06:00 [pubmed]
PHST- 2010/05/21 06:00 [medline]
PST - ppublish
SO  - Int J Mol Med. 2010 Apr;25(4):573-80.