PMID- 20200310
OWN - NLM
STAT- MEDLINE
DCOM- 20100721
LR  - 20220309
IS  - 1935-5548 (Electronic)
IS  - 0149-5992 (Print)
IS  - 0149-5992 (Linking)
VI  - 33
IP  - 5
DP  - 2010 May
TI  - Suppressive effect of insulin infusion on chemokines and chemokine receptors.
PG  - 1103-8
LID - 10.2337/dc09-2193 [doi]
AB  - OBJECTIVE: In view of the previously described anti-inflammatory effects of 
      insulin, we investigated the potential suppressive effect of insulin on plasma 
      concentrations and expression of the chemokines, monocyte chemoattractant 
      protein-1 (MCP-1) and regulated on activation normal T-cell expressed and 
      secreted (RANTES) and their receptors, chemokine receptor (CCR)-2 and CCR-5, in 
      mononuclear cells (MNCs). We also investigated the effect of insulin on other 
      chemokines. RESEARCH DESIGN AND METHODS: Ten obese type 2 diabetic patients were 
      infused with insulin (2 units/h with 100 ml of 5% dextrose/h) for 4 h. Another 8 
      and 6 type 2 diabetic patients were infused with 100 ml of 5% dextrose/h or 
      saline for 4 h, respectively, and served as control subjects. Blood samples were 
      obtained at 0, 2, 4, and 6 h. RESULTS: Insulin infusion significantly suppressed 
      the plasma concentrations of MCP-1, eotaxin, and RANTES and the expression of 
      RANTES, macrophage inflammatory protein (MIP)-1beta, CCR-2, and CCR-5 in MNCs at 
      2 and 4 h. Dextrose and saline infusions did not alter these indexes. 
      CONCLUSIONS: A low-dose infusion of insulin suppresses the plasma concentration 
      of key chemokines, MCP-1, and RANTES, and the expression of their respective 
      receptors, CCR-2 and CCR-5, in MNCs. Insulin also suppresses the expression of 
      RANTES and MIP-1beta in MNCs. These actions probably contribute to the 
      comprehensive anti-inflammatory effect of insulin.
FAU - Ghanim, Husam
AU  - Ghanim H
AD  - Division of Endocrinology, Diabetes and Metabolism, State University of New York 
      at Buffalo, New York, USA. pdandona@kaleidahealth.org
FAU - Korzeniewski, Kelly
AU  - Korzeniewski K
FAU - Sia, Chang Ling
AU  - Sia CL
FAU - Abuaysheh, Sanaa
AU  - Abuaysheh S
FAU - Lohano, Teekam
AU  - Lohano T
FAU - Chaudhuri, Ajay
AU  - Chaudhuri A
FAU - Dandona, Paresh
AU  - Dandona P
LA  - eng
GR  - R01 DK069805/DK/NIDDK NIH HHS/United States
GR  - R01 DK075877/DK/NIDDK NIH HHS/United States
GR  - R01-DK-069805/DK/NIDDK NIH HHS/United States
GR  - R01-DK-075877/DK/NIDDK NIH HHS/United States
PT  - Controlled Clinical Trial
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100303
PL  - United States
TA  - Diabetes Care
JT  - Diabetes care
JID - 7805975
RN  - 0 (Blood Glucose)
RN  - 0 (CCL11 protein, human)
RN  - 0 (CCL4 protein, human)
RN  - 0 (CCR2 protein, human)
RN  - 0 (CX3C Chemokine Receptor 1)
RN  - 0 (CX3CL1 protein, human)
RN  - 0 (CX3CR1 protein, human)
RN  - 0 (CXCL12 protein, human)
RN  - 0 (CXCR4 protein, human)
RN  - 0 (Chemokine CCL11)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokine CX3CL1)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Chemokines)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (Insulin)
RN  - 0 (Interleukin-8)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, CCR5)
RN  - 0 (Receptors, CXCR4)
RN  - 0 (Receptors, Chemokine)
SB  - IM
MH  - Adult
MH  - Blood Glucose/metabolism
MH  - CX3C Chemokine Receptor 1
MH  - Chemokine CCL11/blood/genetics
MH  - Chemokine CCL4/blood/genetics
MH  - Chemokine CCL5/blood/genetics
MH  - Chemokine CX3CL1/blood/genetics
MH  - Chemokine CXCL12/blood/genetics
MH  - Chemokines/*blood/genetics
MH  - Diabetes Mellitus, Type 2/blood/*drug therapy/immunology
MH  - Gene Expression/immunology
MH  - Humans
MH  - Hypoglycemic Agents/administration & dosage/blood
MH  - Immunosuppressive Agents/administration & dosage
MH  - Inflammation/blood/drug therapy/immunology
MH  - Infusions, Intravenous
MH  - Insulin/*administration & dosage/blood
MH  - Interleukin-8/blood/genetics
MH  - Middle Aged
MH  - Obesity/blood/immunology
MH  - Receptors, CCR2/blood/genetics
MH  - Receptors, CCR5/blood/genetics
MH  - Receptors, CXCR4/blood/genetics
MH  - Receptors, Chemokine/*blood/genetics
PMC - PMC2858184
EDAT- 2010/03/05 06:00
MHDA- 2010/07/22 06:00
PMCR- 2011/05/01
CRDT- 2010/03/05 06:00
PHST- 2010/03/05 06:00 [entrez]
PHST- 2010/03/05 06:00 [pubmed]
PHST- 2010/07/22 06:00 [medline]
PHST- 2011/05/01 00:00 [pmc-release]
AID - dc09-2193 [pii]
AID - 2193 [pii]
AID - 10.2337/dc09-2193 [doi]
PST - ppublish
SO  - Diabetes Care. 2010 May;33(5):1103-8. doi: 10.2337/dc09-2193. Epub 2010 Mar 3.