PMID- 2020125
OWN - NLM
STAT- MEDLINE
DCOM- 19910530
LR  - 20151119
IS  - 0047-1879 (Print)
IS  - 0047-1879 (Linking)
VI  - 33
IP  - 1
DP  - 1991 Jan
TI  - [Experimental study on methyl bromide poisoning in mice. Acute inhalation study 
      and the effect of glutathione as an antidote].
PG  - 23-30
AB  - The objectives of this study were to determine the LC50 of methyl bromide (MeBr) 
      and the dose-response curve and to study the detoxication effect of reduced 
      glutathione (GSH) on MeBr poisoning in mice. 1) The LC50 of 4-h exposure to MeBr 
      was 405 ppm in male mice with 95% confidence limits of 386-425 ppm. 2) The 
      mortality rates of mice exposed to 500 ppm MeBr for 105, 120, 130, 140, 150 and 
      180 min were 0, 0, 10.7, 15.0, 85.0 and 90.0%, respectively. 3) In contrast, the 
      mortality rate of mice pretreated with GSH (i.p 500 mg/kg; GSH-group) was only 
      5.3% after exposure to 500 ppm MeBr for 150 min. 4) Metabolic substances (Br-, 
      GSH, formaldehyde, formic acid and beta-glucuronidase) were analyzed after 
      exposure to 500 ppm MeBr and compared with the GSH-group and the distilled water 
      treated group (DW-group). Except for GSH, concentrations of all other substances 
      were significantly lower in the GSH-group than in the DW-group. Erythrocyte 
      osmotic fragility test showed a significant increase in fragility in the 
      DW-group. These results suggested that the onset of symptoms or death due to MeBr 
      poisoning suddenly occurs at some point of concentration and time exposure. It 
      was also shown that pretreatment with GSH effectively reduced mortality due to 
      MeBr exposure.
FAU - Yamano, Y
AU  - Yamano Y
AD  - Department of Hygiene and Public Health, Tokyo Women's Medical College.
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Sangyo Igaku
JT  - Sangyo igaku. Japanese journal of industrial health
JID - 0150531
RN  - 0 (Antidotes)
RN  - 0 (Hydrocarbons, Brominated)
RN  - 9V42E1Z7B6 (methyl bromide)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Administration, Inhalation
MH  - Animals
MH  - Antidotes
MH  - Glutathione/*therapeutic use
MH  - Hydrocarbons, Brominated/administration & dosage/antagonists & 
      inhibitors/*poisoning
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Rats
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
PST - ppublish
SO  - Sangyo Igaku. 1991 Jan;33(1):23-30.