PMID- 2020192
OWN - NLM
STAT- MEDLINE
DCOM- 19910528
LR  - 20190817
IS  - 0022-4804 (Print)
IS  - 0022-4804 (Linking)
VI  - 50
IP  - 4
DP  - 1991 Apr
TI  - Suppression of lymphocyte proliferation through the nitric oxide synthesizing 
      pathway.
PG  - 403-9
AB  - The amino acid L-arginine can be metabolized through a nitric oxide-synthesizing 
      pathway (NOSP) to produce L-citrulline and reactive nitrogen intermediates. Among 
      these nitrogen intermediates, NO has been implicated as the mediator of a variety 
      of biological effects including vasodilatation, inhibition of platelet 
      aggregation, tumor cytotoxicity and microbiostasis by activated macrophages and 
      generalized suppression of macrophage functions. Work reported here demonstrated 
      that the NOSP is expressed in Con A-stimulated rat splenic cell (SC) cultures and 
      is associated with a profound suppression of lymphocyte proliferation. Inhibition 
      of the NOSP by NG-monomethyl-L-arginine (N-MMA) or binding of its products by 
      hemoglobin, either free in solution or contained in RBC, markedly promotes rat SC 
      mitogenic response to Con A. Mouse SC do not express the NOSP under the 
      conditions used in these experiments. Consequently, their mitogenic response to 
      Con A is not affected by N-MMA or hemoglobin. These data confirm and expand the 
      apparent role of NO as a regulator of immune responses while indicating 
      potentially important species differences.
FAU - Albina, J E
AU  - Albina JE
AD  - Department of Surgery, Rhode Island Hospital, Providence 02903.
FAU - Henry, W L Jr
AU  - Henry WL Jr
LA  - eng
GR  - GM-42859/GM/NIGMS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (Hemoglobins)
RN  - 0 (Nitrites)
RN  - 11028-71-0 (Concanavalin A)
RN  - 27JT06E6GR (omega-N-Methylarginine)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 8W8T17847W (Urea)
RN  - 94ZLA3W45F (Arginine)
RN  - EC 3.5.3.1 (Arginase)
SB  - IM
MH  - Animals
MH  - Arginase/metabolism
MH  - Arginine/analogs & derivatives/metabolism/pharmacology
MH  - Cell Adhesion
MH  - Cells, Cultured
MH  - Concanavalin A
MH  - Hemoglobins/metabolism
MH  - In Vitro Techniques
MH  - Lymphocyte Activation/*drug effects
MH  - Lymphocytes/*metabolism
MH  - Mice
MH  - Nitric Oxide/*metabolism
MH  - Nitrites/metabolism
MH  - Rats
MH  - Spleen/cytology
MH  - Urea/metabolism
MH  - omega-N-Methylarginine
EDAT- 1991/04/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1991/04/01 00:00
PHST- 1991/04/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1991/04/01 00:00 [entrez]
AID - 0022-4804(91)90210-D [pii]
AID - 10.1016/0022-4804(91)90210-d [doi]
PST - ppublish
SO  - J Surg Res. 1991 Apr;50(4):403-9. doi: 10.1016/0022-4804(91)90210-d.