PMID- 20203267
OWN - NLM
STAT- MEDLINE
DCOM- 20100525
LR  - 20210206
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Linking)
VI  - 115
IP  - 18
DP  - 2010 May 6
TI  - Delineation of precursors in murine spleen that develop in contact with splenic 
      endothelium to give novel dendritic-like cells.
PG  - 3678-85
LID - 10.1182/blood-2009-06-227108 [doi]
AB  - Hematopoietic cell lineages are best described in terms of distinct progenitors 
      with limited differentiative capacity. To distinguish cell lineages, it is 
      necessary to define progenitors and induce their differentiation in vitro. We 
      previously reported in vitro development of immature dendritic-like cells (DCs) 
      in long-term cultures (LTCs) of murine spleen, and in cocultures of spleen or 
      bone marrow (BM) over splenic endothelial cell lines derived from LTCs. Cells 
      produced are phenotypically distinct CD11b(hi)CD11c(lo)CD8(-)MHC-II(-) cells, 
      tentatively named L-DCs. Here we delineate L-DC progenitors as different from 
      known DC progenitors in BM and DC precursors in spleen. The progenitor is 
      contained within the lineage-negative (Lin)(-)c-kit(+) subset in neonatal and 
      adult spleen. This subset has multipotential reconstituting ability in mice. In 
      neonatal spleen, the progenitor is further enriched within the c-kit(lo) and 
      CD34(+) subsets of Lin(-)c-kit(+) cells. These cells seed cocultures of splenic 
      endothelial cells, differentiating to give L-DCs that can activate T cells. L-DC 
      progenitors are distinguishable from described splenic CD11c(lo) DC precursors 
      and from Fms-like tyrosine kinase 3(+) DC progenitors in BM. Overall, this study 
      confirms that LTCs are a physiologically relevant culture system for in vitro 
      development of a novel DC type from spleen progenitors.
FAU - Tan, Jonathan K H
AU  - Tan JK
AD  - Research School of Biology, Australian National University, Canberra, ACT, 
      Australia, 0200.
FAU - Periasamy, Pravin
AU  - Periasamy P
FAU - O'Neill, Helen C
AU  - O'Neill HC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100304
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Bone Marrow
MH  - Cell Differentiation
MH  - Cell Lineage
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dendritic Cells/*cytology/metabolism
MH  - Endothelium, Vascular/*cytology/metabolism
MH  - Flow Cytometry
MH  - Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Myeloid Progenitor Cells/*cytology/metabolism
MH  - Spleen/*cytology/metabolism
MH  - T-Lymphocytes/metabolism
EDAT- 2010/03/06 06:00
MHDA- 2010/05/26 06:00
CRDT- 2010/03/06 06:00
PHST- 2010/03/06 06:00 [entrez]
PHST- 2010/03/06 06:00 [pubmed]
PHST- 2010/05/26 06:00 [medline]
AID - S0006-4971(20)35053-9 [pii]
AID - 10.1182/blood-2009-06-227108 [doi]
PST - ppublish
SO  - Blood. 2010 May 6;115(18):3678-85. doi: 10.1182/blood-2009-06-227108. Epub 2010 
      Mar 4.