PMID- 20204297
OWN - NLM
STAT- MEDLINE
DCOM- 20100614
LR  - 20161125
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 23
IP  - 4
DP  - 2010 Apr
TI  - Hypomethylation-induced expression of S100A4 increases the invasiveness of 
      laryngeal squamous cell carcinoma.
PG  - 1101-7
AB  - The present study aimed to identify genes related to 5AZA-CdR in laryngeal 
      squamous cell carcinoma (LSCC) and to investigate the role of S100A4 in the 
      development and aggression of LSCC. Differentially expressed proteins were 
      identified in Hep-2 cells treated with 5AZA-CdR by two-dimensional gel 
      electrophoresis combined with MALDI-TOF-MS. mRNA, protein levels and DNA 
      methylation status of S100A4 were assessed by RT-PCR, Western blotting and 
      methylation-specific PCR, respectively. The invasiveness of Hep-2 cells 
      transfected by siRNA S100A4 was determined by transwell migration assay. Protein 
      profiles from Hep-2 cells treated with 5AZA-CdR were obtained, and several 
      differentially expressed proteins such as S100 calcium-binding protein A4 
      (S100A4) were identified. Results of RT-PCR and Western blotting revealed that 
      both mRNA and protein levels of S100A4 were significantly higher in the 
      metastatic lymph nodes than those in paired adjacent normal laryngeal (PANL) or 
      tumor tissues. The DNA methylation status displayed significant differences 
      between the LSCC and the PANL tissues. The expression level of S100A4 decreased 
      in Hep-2 cells undergoing RNA interference of S100A4. The number of cells which 
      crossed the basement membrane filter was significantly lower in the RNAi S100A4 
      group when compared with the number in the control group. The abnormal expression 
      of S100A4 identified in Hep-2 cells treated with an inhibitor of DNA 
      methyltransferase appeared to result from the aberrant DNA methylation status of 
      S100A4. The abnormal expression of S100A4 altered the invasiveness of LSCC.
FAU - Liu, Jia
AU  - Liu J
AD  - Department of Medical Genetics, China Medical University, Shenyang 110001, PR 
      China.
FAU - Guo, Yan
AU  - Guo Y
FAU - Fu, Shuang
AU  - Fu S
FAU - Yang, Min
AU  - Yang M
FAU - Sun, Kai-Lai
AU  - Sun KL
FAU - Fu, Wei-Neng
AU  - Fu WN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (RNA, Small Interfering)
RN  - 0 (S100 Calcium-Binding Protein A4)
RN  - 0 (S100 Proteins)
RN  - 142662-27-9 (S100A4 protein, human)
SB  - IM
MH  - Base Sequence
MH  - Blotting, Western
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - DNA Methylation/*genetics
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Gene Expression
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Molecular Sequence Data
MH  - Neoplasm Invasiveness/*genetics/pathology
MH  - RNA Interference
MH  - RNA, Small Interfering
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - S100 Calcium-Binding Protein A4
MH  - S100 Proteins/biosynthesis/*genetics
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Transfection
EDAT- 2010/03/06 06:00
MHDA- 2010/06/15 06:00
CRDT- 2010/03/06 06:00
PHST- 2010/03/06 06:00 [entrez]
PHST- 2010/03/06 06:00 [pubmed]
PHST- 2010/06/15 06:00 [medline]
PST - ppublish
SO  - Oncol Rep. 2010 Apr;23(4):1101-7.