PMID- 20205277
OWN - NLM
STAT- MEDLINE
DCOM- 20100520
LR  - 20190430
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Print)
IS  - 1007-9327 (Linking)
VI  - 16
IP  - 9
DP  - 2010 Mar 7
TI  - Mechanisms mediating CCK-8S-induced contraction of proximal colon in guinea pigs.
PG  - 1076-85
AB  - AIM: To investigate the effects of sulfated cholecystokinin octapeptide (CCK-8S) 
      on the contractile activity of guinea-pig proximal colon. METHODS: Proximal 
      colonic smooth muscle (PCSM) strips were obtained from adult female guinea pigs 
      and contractile response of PCSM strips was recorded using a polyphysiograph. 
      PCSM cells were isolated by enzymatic digestion. Resting potential (RP), action 
      potential (AP), large conductance potassium channel currents (I(BKCa)) and L-type 
      calcium currents (I(Ca-L)) were recorded by patch-clamp techniques. RESULTS: (1) 
      CCK-8S (10(-7) mol/L) enhanced the mean contractile amplitude of colonic circular 
      muscle and longitudinal muscle (LM) strips by 56.53% + or - 11.92% (P = 0.038) 
      and 65.93% + or - 12.98% (P = 0.019), respectively, as well as the mean frequency 
      of LM by 31.69% + or - 13.58% (P = 0.023), which were significantly attenuated by 
      pretreating strips with devazepide, nifedipine, iberiotoxin, thapsigargin (TG) 
      and BAPTA-AM (BA) respectively; (2) CCK-8S (10(-7) mol/L) increased the AP 
      amplitude by 38.6% + or - 3.2% (P = 0.015), decreased AP duration by 36.9% + or - 
      8.7% (P = 0.026), and depolarized the RP from -61.3 + or - 2.7 mV to -29.8 + or - 
      5.9 mV (P = 0.032); and (3) Compared with the normal control group, CCK-8S 
      (10(-7) mol/L) enhanced the peak current of I(BKCa) by 18.7% + or - 2.1% (from 
      916 + or - 183 pA to 1088 + or - 226 pA; at +60 mV; P = 0.029), which was 
      inhibited by respective pretreatment with iberiotoxin and devazepide. 
      Additionally, CCK-8S (10(-7) mol/L) intensified the peak current of I(Ca-L) by 
      40% (from 60 + or - 8 pA to 84 + or - 11 pA; at +10 mV; P = 0.012), compared to 
      the normal control group, which was apparently suppressed by respective 
      pretreatment with nifedipine, devazepide, TG and BA. In the respective presence 
      of heparin and staurosporine, CCK-8S did not significantly enhance I(BKCa) and 
      I(Ca-L). CONCLUSION: The results suggest that CCK-8S promotes guinea-pig proximal 
      colon contraction by CCK1 receptors, following activation of the inositol 
      triphosphate-protein kinase C signal transduction pathway.
FAU - Zhu, Jie
AU  - Zhu J
AD  - Department of Gastroenterology, Renmin Hospital and Research Center of Structural 
      Biology, Wuhan University, Wuhan 430060, Hubei Province, China.
FAU - Chen, Ling
AU  - Chen L
FAU - Xia, Hong
AU  - Xia H
FAU - Luo, He-Sheng
AU  - Luo HS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (8-sulfocholecystokinin octapeptide)
RN  - 0 (Calcium Channels, L-Type)
RN  - 0 (Gastrointestinal Agents)
RN  - 0 (Large-Conductance Calcium-Activated Potassium Channels)
RN  - 0 (Membrane Transport Modulators)
RN  - 0 (Receptor, Cholecystokinin A)
RN  - 85166-31-0 (Inositol 1,4,5-Trisphosphate)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - M03GIQ7Z6P (Sincalide)
RN  - RWP5GA015D (Potassium)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Action Potentials
MH  - Animals
MH  - Calcium/metabolism
MH  - Calcium Channels, L-Type/drug effects/metabolism
MH  - Colon/*drug effects/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Gastrointestinal Agents/*pharmacology
MH  - Gastrointestinal Motility/*drug effects
MH  - Guinea Pigs
MH  - In Vitro Techniques
MH  - Inositol 1,4,5-Trisphosphate/metabolism
MH  - Large-Conductance Calcium-Activated Potassium Channels/drug effects/metabolism
MH  - Membrane Transport Modulators/pharmacology
MH  - Muscle Contraction/*drug effects
MH  - Muscle, Smooth/*drug effects/metabolism
MH  - Patch-Clamp Techniques
MH  - Potassium/metabolism
MH  - Protein Kinase C/metabolism
MH  - Receptor, Cholecystokinin A/drug effects/metabolism
MH  - Signal Transduction/drug effects
MH  - Sincalide/*analogs & derivatives/pharmacology
MH  - Time Factors
PMC - PMC2835783
EDAT- 2010/03/06 06:00
MHDA- 2010/05/21 06:00
PMCR- 2010/03/07
CRDT- 2010/03/06 06:00
PHST- 2010/03/06 06:00 [entrez]
PHST- 2010/03/06 06:00 [pubmed]
PHST- 2010/05/21 06:00 [medline]
PHST- 2010/03/07 00:00 [pmc-release]
AID - 10.3748/wjg.v16.i9.1076 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2010 Mar 7;16(9):1076-85. doi: 10.3748/wjg.v16.i9.1076.