PMID- 20205720
OWN - NLM
STAT- MEDLINE
DCOM- 20100610
LR  - 20220409
IS  - 1744-8069 (Electronic)
IS  - 1744-8069 (Linking)
VI  - 6
DP  - 2010 Mar 5
TI  - Transient Receptor Potential Vanilloid 1 is essential for cisplatin-induced heat 
      hyperalgesia in mice.
PG  - 15
LID - 10.1186/1744-8069-6-15 [doi]
AB  - BACKGROUND: Cisplatin is primarily used for treatment of ovarian and testicular 
      cancer. Oxaliplatin is the only effective treatment for metastatic colorectal 
      cancer. Both are known to cause dose related, cumulative toxic effects on the 
      peripheral nervous system and thirty to forty percent of cancer patients 
      receiving these agents experience painful peripheral neuropathy. The mechanisms 
      underlying painful platinum-induced neuropathy remain poorly understood. Previous 
      studies have demonstrated important roles for TRPV1, TRPM8, and TRPA1 in 
      inflammation and nerve injury induced pain. RESULTS: In this study, using 
      real-time, reverse transcriptase, polymerase chain reaction (RT-PCR), we analyzed 
      the expression of TRPV1, TRPM8, and TRPA1 induced by cisplatin or oxaliplatin in 
      vitro and in vivo. For in vitro studies, cultured E15 rat dorsal root ganglion 
      (DRG) neurons were treated for up to 48 hours with cisplatin or oxaliplatin. For 
      in vivo studies, trigeminal ganglia (TG) were isolated from mice treated with 
      platinum drugs for three weeks. We show that cisplatin and oxaliplatin-treated 
      DRG neurons had significantly increased in TRPV1, TRPA1, and TRPM8 mRNA 
      expression. TG neurons from cisplatin treated mice had significant increases in 
      TRPV1 and TRPA1 mRNA expression while oxaliplatin strongly induced only TRPA1. 
      Furthermore, compared to the cisplatin-treated wild-type mice, cisplatin-treated 
      TRPV1-null mice developed mechanical allodynia but did not exhibit enhancement of 
      noxious heat- evoked pain responses. Immunohistochemistry studies showed that 
      cisplatin-treated mice had no change in the proportion of the TRPV1 
      immunopositive TG neurons. CONCLUSION: These results indicate that TRPV1 and 
      TRPA1 could contribute to the development of thermal hyperalgesia and mechanical 
      allodynia following cisplatin-induced painful neuropathy but that TRPV1 has a 
      crucial role in cisplatin-induced thermal hyperalgesia in vivo.
FAU - Ta, Lauren E
AU  - Ta LE
AD  - Program in Molecular Neuroscience, Mayo Graduate School, Mayo Clinic, College of 
      Medicine, Rochester, MN 55905, USA. ta.lauren@mayo.edu
FAU - Bieber, Allan J
AU  - Bieber AJ
FAU - Carlton, Susan M
AU  - Carlton SM
FAU - Loprinzi, Charles L
AU  - Loprinzi CL
FAU - Low, Philip A
AU  - Low PA
FAU - Windebank, Anthony J
AU  - Windebank AJ
LA  - eng
GR  - CA 124477/CA/NCI NIH HHS/United States
GR  - K05 CA124477/CA/NCI NIH HHS/United States
GR  - R01 NS 40471-04/NS/NINDS NIH HHS/United States
GR  - K08 DE14571-05/DE/NIDCR NIH HHS/United States
GR  - R01 NS 27910-20/NS/NINDS NIH HHS/United States
GR  - R01 NS 48357-03/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20100305
PL  - United States
TA  - Mol Pain
JT  - Molecular pain
JID - 101242662
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Organoplatinum Compounds)
RN  - 0 (RNA, Messenger)
RN  - 0 (TRPA1 Cation Channel)
RN  - 0 (TRPM Cation Channels)
RN  - 0 (TRPM8 protein, mouse)
RN  - 0 (TRPV Cation Channels)
RN  - 0 (TRPV1 protein, mouse)
RN  - 0 (Transient Receptor Potential Channels)
RN  - 0 (Trpa1 protein, mouse)
RN  - 04ZR38536J (Oxaliplatin)
RN  - Q20Q21Q62J (Cisplatin)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/toxicity
MH  - Cells, Cultured
MH  - Cisplatin/*toxicity
MH  - Ganglia, Spinal/drug effects/metabolism
MH  - Hyperalgesia/*chemically induced/*metabolism/physiopathology
MH  - Immunohistochemistry
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Nociceptors/drug effects/metabolism
MH  - Organoplatinum Compounds/toxicity
MH  - Oxaliplatin
MH  - Peripheral Nervous System Diseases/*chemically 
      induced/*metabolism/physiopathology
MH  - RNA, Messenger/drug effects/metabolism
MH  - TRPA1 Cation Channel
MH  - TRPM Cation Channels/drug effects/metabolism
MH  - TRPV Cation Channels/drug effects/genetics/*metabolism
MH  - Transient Receptor Potential Channels/drug effects/metabolism
PMC - PMC2848188
EDAT- 2010/03/09 06:00
MHDA- 2010/06/11 06:00
PMCR- 2010/03/05
CRDT- 2010/03/09 06:00
PHST- 2009/06/24 00:00 [received]
PHST- 2010/03/05 00:00 [accepted]
PHST- 2010/03/09 06:00 [entrez]
PHST- 2010/03/09 06:00 [pubmed]
PHST- 2010/06/11 06:00 [medline]
PHST- 2010/03/05 00:00 [pmc-release]
AID - 1744-8069-6-15 [pii]
AID - 10.1186/1744-8069-6-15 [doi]
PST - epublish
SO  - Mol Pain. 2010 Mar 5;6:15. doi: 10.1186/1744-8069-6-15.