PMID- 20207161
OWN - NLM
STAT- MEDLINE
DCOM- 20100730
LR  - 20100507
IS  - 1096-0023 (Electronic)
IS  - 1043-4666 (Linking)
VI  - 50
IP  - 3
DP  - 2010 Jun
TI  - Transforming growth factor beta-1 and interleukin-17 gene transcription in 
      peripheral blood mononuclear cells and the human response to infection.
PG  - 322-7
LID - 10.1016/j.cyto.2010.01.003 [doi]
AB  - INTRODUCTION: The occurrence of severe sepsis may be associated with deficient 
      pro-inflammatory cytokine production. Transforming growth factor beta-1 
      (TGFbeta-1) predominantly inhibits inflammation and may simultaneously promote 
      IL-17 production. Interleukin-17 (IL-17) is a recently described pro-inflammatory 
      cytokine, which may be important in auto-immunity and infection. We investigated 
      the hypothesis that the onset of sepsis is related to differential TGFbeta-1 and 
      IL-17 gene expression. METHODS: A prospective observational study in a mixed 
      intensive care unit (ICU) and hospital wards in a university hospital. Patients 
      (59) with severe sepsis; 15 patients with gram-negative bacteraemia but without 
      critical illness and 10 healthy controls were assayed for TGFbeta-1, IL-17a, 
      IL-17f, IL-6 and IL-1beta mRNA in peripheral blood mononuclear cells (PBMC) by 
      quantitative real-time PCR and serum protein levels by ELISA. RESULTS: TGFbeta-1 
      mRNA levels are reduced in patients with bacteraemia and sepsis compared with 
      controls (p=0.02). IL-6 mRNA levels were reduced in bacteraemic patients compared 
      with septic patients and controls (p=0.008). IL-1beta mRNA levels were similar in 
      all groups, IL-17a and IL-17f mRNA levels are not detectable in peripheral blood 
      mononuclear cells. IL-6 protein levels were greater in patients with sepsis than 
      bacteraemic and control patients (p<0.0001). Activated TGFbeta-1 and IL-17 
      protein levels were similar in all groups. IL-1beta protein was not detectable in 
      the majority of patients. CONCLUSIONS: Down regulation of TGFbeta-1 gene 
      transcription was related to the occurrence of infection but not the onset of 
      sepsis. Interleukin-17 production in PBMC may not be significant in the human 
      host response to infection.
CI  - Copyright 2010 Elsevier Ltd. All rights reserved.
FAU - White, Mary
AU  - White M
AD  - Department of Anaesthesia, St. James Hospital, Dublin 8, Ireland; Institute of 
      Molecular Medicine, Trinity College Dublin, Ireland. drmbwhite@yahoo.co.uk
FAU - Lawless, Matthew W
AU  - Lawless MW
FAU - O'Dwyer, Michael J
AU  - O'Dwyer MJ
FAU - Grealy, Robert
AU  - Grealy R
FAU - Connell, Brian O
AU  - Connell BO
FAU - Stordeur, Patrick
AU  - Stordeur P
FAU - Kelleher, Dermot
AU  - Kelleher D
FAU - McManus, Ross
AU  - McManus R
FAU - Ryan, Thomas
AU  - Ryan T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100305
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Adrenal Cortex Hormones)
RN  - 0 (IL17A protein, human)
RN  - 0 (Interleukin-17)
RN  - 0 (RNA, Messenger)
RN  - 0 (TGFB1 protein, human)
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Adrenal Cortex Hormones/therapeutic use
MH  - Aged
MH  - Aged, 80 and over
MH  - Bacteremia/genetics/immunology
MH  - Case-Control Studies
MH  - Demography
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Gene Dosage/genetics
MH  - Gene Expression Regulation
MH  - Humans
MH  - Interleukin-17/*blood/*genetics
MH  - Leukocytes, Mononuclear/*metabolism
MH  - Male
MH  - RNA, Messenger/genetics/metabolism
MH  - Sepsis/drug therapy/*genetics
MH  - *Transcription, Genetic
MH  - Transforming Growth Factor beta1/*blood/*genetics
EDAT- 2010/03/09 06:00
MHDA- 2010/07/31 06:00
CRDT- 2010/03/09 06:00
PHST- 2009/07/22 00:00 [received]
PHST- 2009/12/16 00:00 [revised]
PHST- 2010/01/10 00:00 [accepted]
PHST- 2010/03/09 06:00 [entrez]
PHST- 2010/03/09 06:00 [pubmed]
PHST- 2010/07/31 06:00 [medline]
AID - S1043-4666(10)00006-2 [pii]
AID - 10.1016/j.cyto.2010.01.003 [doi]
PST - ppublish
SO  - Cytokine. 2010 Jun;50(3):322-7. doi: 10.1016/j.cyto.2010.01.003. Epub 2010 Mar 5.