PMID- 20207200
OWN - NLM
STAT- MEDLINE
DCOM- 20100609
LR  - 20181201
IS  - 1521-7035 (Electronic)
IS  - 1521-6616 (Linking)
VI  - 135
IP  - 3
DP  - 2010 Jun
TI  - Human umbilical cord mesenchymal stem cells hUC-MSCs exert immunosuppressive 
      activities through a PGE2-dependent mechanism.
PG  - 448-58
LID - 10.1016/j.clim.2010.01.015 [doi]
AB  - Human umbilical-cord-derived mesenchymal stem cells (hUC-MSCs) constitute an 
      attractive alternative to bone-marrow-derived MSCs for potential clinical 
      applications because of easy preparation and lower risk of viral contamination. 
      In this study, both proliferation of human peripheral blood mononuclear cells 
      (hPBMCs) and their IFN-gamma production in response to mitogenic or allogeneic 
      stimulus were effectively inhibited by hUC-MSCs. Co-culture experiments in 
      transwell systems indicated that the suppression was largely mediated by soluble 
      factor(s). Blocking experiments identified prostaglandin E(2) (PGE(2)) as the 
      major factor, because inhibition of PGE(2) synthesis almost completely mitigated 
      the immunosuppressive effects, whereas neutralization of TGF-beta, IDO, and NO 
      activities had little effects. Moreover, the inflammatory cytokines, IFN-gamma 
      and IL-1beta, produced by hPBMCs upon activation notably upregulated the 
      expression of cyclooxygenase-2 (COX-2) and the production of PGE(2) by hUC-MSCs. 
      In conclusion, our data have demonstrated for the first time the PGE(2)-mediated 
      mechanism by which hUC-MSCs exert their immunomodulatory effects.
CI  - Copyright 2010 Elsevier Inc. All rights reserved.
FAU - Chen, Ke
AU  - Chen K
AD  - The State Key Laboratory of Experimental Hematology, Institute of Hematology and 
      Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union 
      of Medical College, Tianjin 300020, China.
FAU - Wang, Ding
AU  - Wang D
FAU - Du, Wei Ting
AU  - Du WT
FAU - Han, Zhi-Bo
AU  - Han ZB
FAU - Ren, He
AU  - Ren H
FAU - Chi, Ying
AU  - Chi Y
FAU - Yang, Shao Guang
AU  - Yang SG
FAU - Zhu, Delin
AU  - Zhu D
FAU - Bayard, Francis
AU  - Bayard F
FAU - Han, Zhong Chao
AU  - Han ZC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100305
PL  - United States
TA  - Clin Immunol
JT  - Clinical immunology (Orlando, Fla.)
JID - 100883537
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Cell Proliferation
MH  - Cell Separation
MH  - Coculture Techniques
MH  - Cyclooxygenase 2/immunology/metabolism
MH  - Cytokines/biosynthesis/immunology
MH  - Dinoprostone/*immunology/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fetal Blood/cytology/immunology/metabolism
MH  - Flow Cytometry
MH  - Gene Expression/immunology
MH  - Humans
MH  - Immune Tolerance/*immunology
MH  - Immunophenotyping
MH  - Interferon-gamma/biosynthesis/immunology
MH  - Leukocytes, Mononuclear/*immunology
MH  - Mesenchymal Stem Cells/cytology/*immunology/metabolism
MH  - RNA, Messenger/analysis
MH  - Umbilical Cord/cytology/immunology/metabolism
EDAT- 2010/03/09 06:00
MHDA- 2010/06/10 06:00
CRDT- 2010/03/09 06:00
PHST- 2009/11/06 00:00 [received]
PHST- 2010/01/21 00:00 [revised]
PHST- 2010/01/27 00:00 [accepted]
PHST- 2010/03/09 06:00 [entrez]
PHST- 2010/03/09 06:00 [pubmed]
PHST- 2010/06/10 06:00 [medline]
AID - S1521-6616(10)00036-7 [pii]
AID - 10.1016/j.clim.2010.01.015 [doi]
PST - ppublish
SO  - Clin Immunol. 2010 Jun;135(3):448-58. doi: 10.1016/j.clim.2010.01.015. Epub 2010 
      Mar 5.