PMID- 20211207
OWN - NLM
STAT- MEDLINE
DCOM- 20100712
LR  - 20100420
IS  - 1872-8359 (Electronic)
IS  - 0167-7012 (Linking)
VI  - 81
IP  - 2
DP  - 2010 May
TI  - Visualization of initial bacterial colonization on dentine and enamel in situ.
PG  - 166-74
LID - 10.1016/j.mimet.2010.03.002 [doi]
AB  - Bacterial colonization of dentine is of high relevance in cariology, 
      endodontology and periodontology. The aim of the present in situ study was to 
      establish recent methods for visualization and quantification of initial 
      bacterial adherence to dentine in comparison to enamel. For this purpose, bovine 
      enamel and dentine slabs were fixed on buccal sites of individual upper jaw 
      splints worn by 6 subjects for 30min, 120min and 360min, respectively. Adherent 
      bacteria on the slabs were visualized and quantified with DAPI-staining 
      (4',6-diamidino-2-phenylindole) and fluorescence in situ hybridization (FISH) of 
      streptococci and eubacteria using the CLSM (confocal laser scanning microscopy) 
      as well as an epifluorescence microscope. In addition, the number of colony 
      forming units was quantified after desorption. Representative samples were 
      processed for SEM (scanning electron microscopy) and TEM (transmission electron 
      microscopy). All methods clearly indicated that a significantly higher number of 
      bacteria adhered to dentine than to enamel. Furthermore, the amount of bacteria 
      on the dentine increased with increasing oral exposure time, but remained rather 
      constant on the enamel. The CLSM allowed visualization of bacteria in the 
      dentinal tubules. Bacteria were found preferentially at the openings of the 
      dentine tubules, but were distributed randomly on the enamel. In conclusion, the 
      adopted methods are suitable for visualization and quantification of bacterial 
      adhesion to dentine. Even the initial bacterial colonization of dentine is much 
      more pronounced than bacterial adherence to the enamel.
CI  - Copyright 2010 Elsevier B.V. All rights reserved.
FAU - Jung, David Jonathan
AU  - Jung DJ
AD  - Department of Operative Dentistry and Periodontology, University of Freiburg, 
      Hugstetter Str. 55, D-79106 Freiburg, Germany.
FAU - Al-Ahmad, Ali
AU  - Al-Ahmad A
FAU - Follo, Marie
AU  - Follo M
FAU - Spitzmuller, Bettina
AU  - Spitzmuller B
FAU - Hoth-Hannig, Wiebke
AU  - Hoth-Hannig W
FAU - Hannig, Matthias
AU  - Hannig M
FAU - Hannig, Christian
AU  - Hannig C
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100306
PL  - Netherlands
TA  - J Microbiol Methods
JT  - Journal of microbiological methods
JID - 8306883
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indoles)
RN  - 47165-04-8 (DAPI)
SB  - IM
MH  - Animals
MH  - Bacteria/*growth & development
MH  - *Bacterial Adhesion
MH  - *Bacterial Physiological Phenomena
MH  - Cattle
MH  - Dental Enamel/*microbiology
MH  - Dentin/*microbiology
MH  - Fluorescent Dyes/pharmacology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Indoles/pharmacology
MH  - Microscopy, Electron, Scanning
MH  - Staining and Labeling/methods
MH  - Time Factors
EDAT- 2010/03/10 06:00
MHDA- 2010/07/14 06:00
CRDT- 2010/03/10 06:00
PHST- 2009/11/03 00:00 [received]
PHST- 2010/02/25 00:00 [revised]
PHST- 2010/03/01 00:00 [accepted]
PHST- 2010/03/10 06:00 [entrez]
PHST- 2010/03/10 06:00 [pubmed]
PHST- 2010/07/14 06:00 [medline]
AID - S0167-7012(10)00088-6 [pii]
AID - 10.1016/j.mimet.2010.03.002 [doi]
PST - ppublish
SO  - J Microbiol Methods. 2010 May;81(2):166-74. doi: 10.1016/j.mimet.2010.03.002. 
      Epub 2010 Mar 6.