PMID- 20211581
OWN - NLM
STAT- MEDLINE
DCOM- 20100714
LR  - 20181113
IS  - 1873-5967 (Electronic)
IS  - 1386-6532 (Print)
IS  - 1386-6532 (Linking)
VI  - 48
IP  - 1
DP  - 2010 May
TI  - Diagnostic assays for active infection with human herpesvirus 6 (HHV-6).
PG  - 55-7
LID - 10.1016/j.jcv.2010.02.007 [doi]
AB  - BACKGROUND: Human herpesvirus 6 (HHV-6) causes ubiquitous infection in early 
      childhood with lifelong latency or persistence. Reactivation of HHV-6 has been 
      associated with multiple diseases including encephalitis. Chromosomal integration 
      of HHV-6 also occurs. Previous studies have suggested that the detection of HHV-6 
      DNA in plasma is an accurate marker of active viral replication. OBJECTIVE: We 
      sought to determine whether PCR assays on plasma could correctly differentiate 
      between primary HHV-6 infection, chromosomal integration of HHV-6 and latent 
      HHV-6 infection. STUDY DESIGN: We performed qualitative PCR, real-time 
      quantitative PCR (RQ-PCR), and reverse-transcriptase PCR (RT-PCR) assays on 
      samples of peripheral and cord blood mononuclear cells, as well as plasma, from 
      groups of subjects with well defined HHV-6 infection, including subjects with 
      chromosomally integrated HHV-6. RESULTS AND CONCLUSIONS: The detection of HHV-6 
      DNA in plasma was 92% sensitive compared to viral isolation for the 
      identification of primary infection with HHV-6. All plasma samples from infants 
      with chromosomally integrated HHV-6 had HHV-6 DNA detectable in plasma while only 
      5.6% were positive by RT-PCR. The specificity of plasma PCR for active 
      replication of HHV-6 was 84% compared to viral culture while the specificity of 
      RT-PCR was 98%. Our results demonstrate that qualitative or quantitative PCR of 
      plasma is insufficient to distinguish between active viral replication and 
      chromosomal integration with HHV-6. We found a higher specificity of RT-PCR 
      performed on PBMC samples compared to PCR or RQ-PCR performed on plasma when 
      evaluating samples for active HHV-6 replication.
CI  - Copyright 2010 Elsevier B.V. All rights reserved.
FAU - Caserta, Mary T
AU  - Caserta MT
AD  - Department of Pediatrics, University of Rochester School of Medicine and 
      Dentistry, Rochester, NY 14642, United States. mary_caserta@urmc.rochester.edu
FAU - Hall, Caroline Breese
AU  - Hall CB
FAU - Schnabel, Kenneth
AU  - Schnabel K
FAU - Lofthus, Geraldine
AU  - Lofthus G
FAU - Marino, Andrea
AU  - Marino A
FAU - Shelley, Lynne
AU  - Shelley L
FAU - Yoo, Christina
AU  - Yoo C
FAU - Carnahan, Jennifer
AU  - Carnahan J
FAU - Anderson, Linda
AU  - Anderson L
FAU - Wang, Hongyue
AU  - Wang H
LA  - eng
GR  - 5R01 AI 33020-09/AI/NIAID NIH HHS/United States
GR  - 5 MO1 RR00044/RR/NCRR NIH HHS/United States
GR  - M01 RR000044/RR/NCRR NIH HHS/United States
GR  - M01 RR000044-440762/RR/NCRR NIH HHS/United States
GR  - R01 AI033020-09/AI/NIAID NIH HHS/United States
GR  - M01 RR000044-430446/RR/NCRR NIH HHS/United States
GR  - R01 HD044430/HD/NICHD NIH HHS/United States
GR  - R01 HD 44430-01/HD/NICHD NIH HHS/United States
GR  - R01 HD044430-01/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20100307
PL  - Netherlands
TA  - J Clin Virol
JT  - Journal of clinical virology : the official publication of the Pan American 
      Society for Clinical Virology
JID - 9815671
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Child
MH  - DNA, Viral/blood
MH  - Herpesvirus 6, Human/genetics/*isolation & purification/physiology
MH  - Humans
MH  - Polymerase Chain Reaction/methods
MH  - Roseolovirus Infections/blood/diagnosis/*virology
MH  - Sensitivity and Specificity
MH  - Virology/methods
MH  - Virus Integration
MH  - Virus Replication
PMC - PMC2855742
MID - NIHMS182142
COIS- The authors have no conflict of interest.
EDAT- 2010/03/10 06:00
MHDA- 2010/07/16 06:00
PMCR- 2011/05/01
CRDT- 2010/03/10 06:00
PHST- 2009/11/03 00:00 [received]
PHST- 2010/02/10 00:00 [revised]
PHST- 2010/02/11 00:00 [accepted]
PHST- 2010/03/10 06:00 [entrez]
PHST- 2010/03/10 06:00 [pubmed]
PHST- 2010/07/16 06:00 [medline]
PHST- 2011/05/01 00:00 [pmc-release]
AID - S1386-6532(10)00058-2 [pii]
AID - 10.1016/j.jcv.2010.02.007 [doi]
PST - ppublish
SO  - J Clin Virol. 2010 May;48(1):55-7. doi: 10.1016/j.jcv.2010.02.007. Epub 2010 Mar 
      7.