PMID- 20213042
OWN - NLM
STAT- MEDLINE
DCOM- 20100609
LR  - 20190513
IS  - 1745-7270 (Electronic)
IS  - 1672-9145 (Linking)
VI  - 42
IP  - 3
DP  - 2010 Mar 15
TI  - Autophagy promotes BCG-induced maturation of human dendritic cells.
PG  - 177-82
AB  - Dendritic cells (DCs) are important for the initiation of the adaptive immune 
      response against Mycobacterium tuberculosis. Autophagy is an innate and adaptive 
      defense mechanism and important for the control of M. tuberculosis. However, the 
      role of autophagy in the adaptive immune response against M. tuberculosis remains 
      to be determined. In the present study, we studied the effects of autophagy on 
      the maturation of DCs infected with Bacillus Calmette- Guerin (BCG). The 
      phenotype and function of the DCs were assessed by measuring the expression of 
      CD86 and HLA-DR and the secretion of IL-10 and IL-6. Autophagy was evaluated by 
      the change in LC3II, a molecular marker for autophagy. Following stimulation of 
      autophagy, DCs that were matured in the presence of BCG showed enhanced 
      expression of CD86 and HLA-DR and increased IL-6 production. The expression of 
      LC3II was increased after the stimulation of autophagy. These results 
      demonstrated that autophagy might result in the increased maturation of 
      BCG-infected DCs, suggesting that autophagy could contribute to an enhanced 
      adaptive immune response against M. tuberculosis.
FAU - Min, Yan
AU  - Min Y
AD  - State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan 
      University, Shanghai 200433, China.
FAU - Xu, Wenxi
AU  - Xu W
FAU - Liu, Dan
AU  - Liu D
FAU - Shen, Suqin
AU  - Shen S
FAU - Lu, Yunzhi
AU  - Lu Y
FAU - Zhang, Lu
AU  - Zhang L
FAU - Wang, Honghai
AU  - Wang H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Acta Biochim Biophys Sin (Shanghai)
JT  - Acta biochimica et biophysica Sinica
JID - 101206716
RN  - 0 (Cytokines)
SB  - IM
MH  - Autophagy/*immunology
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Cytokines/*immunology
MH  - Dendritic Cells/*immunology/*microbiology
MH  - Humans
MH  - Mycobacterium bovis/*physiology
EDAT- 2010/03/10 06:00
MHDA- 2010/06/10 06:00
CRDT- 2010/03/10 06:00
PHST- 2010/03/10 06:00 [entrez]
PHST- 2010/03/10 06:00 [pubmed]
PHST- 2010/06/10 06:00 [medline]
AID - 10.1093/abbs/gmq006 [doi]
PST - ppublish
SO  - Acta Biochim Biophys Sin (Shanghai). 2010 Mar 15;42(3):177-82. doi: 
      10.1093/abbs/gmq006.