PMID- 20213282
OWN - NLM
STAT- MEDLINE
DCOM- 20100413
LR  - 20191210
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 155
IP  - 4
DP  - 2010 Apr
TI  - A dual effect of porcine reproductive and respiratory syndrome virus replication 
      on the phosphatidylinositol-3-kinase-dependent Akt pathway.
PG  - 571-5
LID - 10.1007/s00705-010-0611-6 [doi]
AB  - We have recently shown that porcine reproductive and respiratory syndrome virus 
      (PRRSV) can undergo a productive replication in porcine monocyte-derived 
      dendritic cells (Mo-DCs). Here, we further demonstrate that PRRSV activates the 
      host's phosphatidylinositol-3-kinase (PI3K)-dependent Akt pathway (PI3K/Akt) to 
      facilitate its replication in Mo-DCs at 90 min and 4 h after infection. 
      Inhibition of PI3K/Akt by treatment with a PI3K-specific inhibitor (LY294002) 
      prior to PRRSV infection reduced virus replication. Furthermore, inhibition of 
      PI3K/Akt by LY294002 at 90 min and 8 h after virus infection still significantly 
      reduced virus production, suggesting that virus replication may be dependent on 
      the activation of PI3K/Akt. Interestingly, PRRSV inhibited PI3K/Akt at 12 h after 
      infection. Heat-inactivated virus failed to inhibit PI3K/Akt, indicating that 
      virus replication is essential for this inhibition. Overall, PRRSV replication 
      exhibits a dual effect on the PI3K/Akt pathway in which both time-dependent 
      activation and inhibition of PI3K/Akt are observed.
FAU - Zhang, Hanmo
AU  - Zhang H
AD  - Department of Biology and Microbiology, South Dakota State University, NPB 252D, 
      Box 2140D, Brookings, SD 57007, USA.
FAU - Wang, Xiuqing
AU  - Wang X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20100307
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Chromones)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Morpholines)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 31M2U1DVID (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Chromones/pharmacology
MH  - Dendritic Cells/*virology
MH  - Enzyme Inhibitors/pharmacology
MH  - Morpholines/pharmacology
MH  - Phosphatidylinositol 3-Kinases/*metabolism
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - *Signal Transduction
MH  - Swine
MH  - Viral Load
MH  - *Virus Replication
EDAT- 2010/03/10 06:00
MHDA- 2010/04/14 06:00
CRDT- 2010/03/10 06:00
PHST- 2009/09/04 00:00 [received]
PHST- 2010/01/08 00:00 [accepted]
PHST- 2010/03/10 06:00 [entrez]
PHST- 2010/03/10 06:00 [pubmed]
PHST- 2010/04/14 06:00 [medline]
AID - 10.1007/s00705-010-0611-6 [doi]
PST - ppublish
SO  - Arch Virol. 2010 Apr;155(4):571-5. doi: 10.1007/s00705-010-0611-6. Epub 2010 Mar 
      7.