PMID- 20214886
OWN - NLM
STAT- MEDLINE
DCOM- 20100504
LR  - 20100412
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 394
IP  - 3
DP  - 2010 Apr 9
TI  - Different effects of 25-kDa amelogenin on the proliferation, attachment and 
      migration of various periodontal cells.
PG  - 581-6
LID - 10.1016/j.bbrc.2010.03.023 [doi]
AB  - Previous studies have assumed that amelogenin is responsible for the therapeutic 
      effect of the enamel matrix derivative (EMD) in periodontal tissue healing and 
      regeneration. However, it is difficult to confirm this hypothesis because both 
      the EMD and the amelogenins are complex mixtures of multiple proteins. Further 
      adding to the difficulties is the fact that periodontal tissue regeneration 
      involves various types of cells and a sequence of associated cellular events 
      including the attachment, migration and proliferation of various cells. In this 
      study, we investigated the potential effect of a 25-kDa recombinant porcine 
      amelogenin (rPAm) on primarily cultured periodontal ligament fibroblasts (PDLF), 
      gingival fibroblasts (GF) and gingival epithelial cells (GEC). The cells were 
      treated with 25-kDa recombinant porcine amelogenin at a concentration of 10 
      microg/mL. We found that rPAm significantly promoted the proliferation and 
      migration of PDLF, but not their adhesion. Similarly, the proliferation and 
      adhesion of GF were significantly enhanced by treatment with rPAm, while 
      migration was greatly inhibited. Interestingly, this recombinant protein 
      inhibited the growth rate, cell adhesion and migration of GEC. These data suggest 
      that rPAm may play an essential role in periodontal regeneration through the 
      activation of periodontal fibroblasts and inhibition of the cellular behaviors of 
      gingival epithelial cells.
CI  - Copyright 2010 Elsevier Inc. All rights reserved.
FAU - Li, Xiting
AU  - Li X
AD  - Department of Periodontology, Ninth People's Hospital, Shanghai Jiao Tong 
      University, School of Medicine, Shanghai Key Laboratory of Stomatology, No. 639 
      Zhi Zao Ju Road, Shanghai 200011, China.
FAU - Shu, Rong
AU  - Shu R
FAU - Liu, Dali
AU  - Liu D
FAU - Jiang, Shaoyun
AU  - Jiang S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100307
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Amelogenin)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Amelogenin/genetics/pharmacology/*physiology
MH  - Animals
MH  - Cell Adhesion
MH  - *Cell Movement
MH  - *Cell Proliferation
MH  - Cells, Cultured
MH  - Fibroblasts/drug effects/physiology
MH  - Humans
MH  - Periodontal Ligament/cytology/drug effects/*physiology
MH  - Rats
MH  - Recombinant Proteins/genetics/pharmacology
MH  - *Regeneration
MH  - Swine
EDAT- 2010/03/11 06:00
MHDA- 2010/05/05 06:00
CRDT- 2010/03/11 06:00
PHST- 2010/02/20 00:00 [received]
PHST- 2010/03/04 00:00 [accepted]
PHST- 2010/03/11 06:00 [entrez]
PHST- 2010/03/11 06:00 [pubmed]
PHST- 2010/05/05 06:00 [medline]
AID - S0006-291X(10)00460-2 [pii]
AID - 10.1016/j.bbrc.2010.03.023 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2010 Apr 9;394(3):581-6. doi: 
      10.1016/j.bbrc.2010.03.023. Epub 2010 Mar 7.