PMID- 20215408
OWN - NLM
STAT- MEDLINE
DCOM- 20100611
LR  - 20131121
IS  - 1521-0103 (Electronic)
IS  - 0022-3565 (Linking)
VI  - 333
IP  - 3
DP  - 2010 Jun
TI  - The herbal medicine compound falcarindiol from Notopterygii Rhizoma suppresses 
      dendritic cell maturation.
PG  - 954-60
LID - 10.1124/jpet.109.162305 [doi]
AB  - Dendritic cells (DCs) are important for regulating the immune response. We report 
      an herbal medicine compound called falcarindiol that affects DC function. Ethanol 
      extracts of 99 crude drugs that are the main components of 210 traditional 
      Japanese medicines (Kampo medicine) approved by the Ministry of Health, Labor and 
      Welfare in Japan were prepared and screened using the murine epidermal-derived 
      Langerhans cell line XS106. Notopterygii Rhizoma strongly suppressed major 
      histocompatibility complex (MHC) class II expression in XS106 cells. 
      Activity-guided fractionation led to the isolation and identification of 
      falcarindiol as a principal active compound in Notopterygii Rhizoma. Falcarindiol 
      (1-5 microM) dose-dependently suppressed MHC II expression in XS106 cells. 
      Fresh-isolated bone marrow-derived DCs were examined for the production of MHC 
      II, CD80, CD86, interleukin (IL)-12p70, and IL-10. Treatment of bone 
      marrow-derived DCs with 5 muM falcarindiol significantly inhibited 
      lipopolysaccharide-induced phenotype activation and cytokine secretion and 
      inhibited MHC II expression by CD40 ligation, but not phorbol 12-myristate 
      13-acetate + ionomycin or IL-12. Falcarindiol inhibited DC maturation by blocking 
      the canonical pathway of nuclear factor-kappaB and phosphorylated p38. Topical 
      application of 0.002 and 0.01% falcarindiol before sensitization dose-dependently 
      suppressed delayed-type hypersensitivity to ovalbumin (p < 0.01). Falcarindiol 
      induces immunosuppressive effects in vitro and in vivo and might be a novel 
      therapy for autoimmune or allergic diseases.
FAU - Mitsui, Seika
AU  - Mitsui S
AD  - Department of Geriatric and Environmental Dermatology, Nagoya City University 
      Graduate School of Medical Sciences, Mizuho-ku, Nagoya, Japan.
FAU - Torii, Kan
AU  - Torii K
FAU - Fukui, Hajime
AU  - Fukui H
FAU - Tsujimura, Kunio
AU  - Tsujimura K
FAU - Maeda, Akira
AU  - Maeda A
FAU - Nose, Mitsuhiko
AU  - Nose M
FAU - Nagatsu, Akito
AU  - Nagatsu A
FAU - Mizukami, Hajime
AU  - Mizukami H
FAU - Morita, Akimichi
AU  - Morita A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100309
PL  - United States
TA  - J Pharmacol Exp Ther
JT  - The Journal of pharmacology and experimental therapeutics
JID - 0376362
RN  - 0 (Cytokines)
RN  - 0 (Diynes)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Fatty Alcohols)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (NF-kappa B)
RN  - 0 (Plant Extracts)
RN  - 55297-87-5 (falcarindiol)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Administration, Topical
MH  - Animals
MH  - Apiaceae/*chemistry
MH  - Bone Marrow Cells/drug effects
MH  - Cell Line
MH  - Cytokines/biosynthesis
MH  - Dendritic Cells/*drug effects
MH  - Diynes/administration & dosage/isolation & purification/*pharmacology
MH  - Drugs, Chinese Herbal
MH  - Fatty Alcohols/administration & dosage/isolation & purification/*pharmacology
MH  - Female
MH  - Flow Cytometry
MH  - Fluorescein-5-isothiocyanate
MH  - Fluorescent Dyes
MH  - Hypersensitivity, Delayed/prevention & control
MH  - Japan
MH  - Magnetic Resonance Spectroscopy
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - NF-kappa B/metabolism
MH  - Plant Extracts/chemistry/pharmacology
MH  - Protein Transport
EDAT- 2010/03/11 06:00
MHDA- 2010/06/12 06:00
CRDT- 2010/03/11 06:00
PHST- 2010/03/11 06:00 [entrez]
PHST- 2010/03/11 06:00 [pubmed]
PHST- 2010/06/12 06:00 [medline]
AID - jpet.109.162305 [pii]
AID - 10.1124/jpet.109.162305 [doi]
PST - ppublish
SO  - J Pharmacol Exp Ther. 2010 Jun;333(3):954-60. doi: 10.1124/jpet.109.162305. Epub 
      2010 Mar 9.