PMID- 20216077
OWN - NLM
STAT- MEDLINE
DCOM- 20110201
LR  - 20220129
IS  - 1539-2031 (Electronic)
IS  - 0192-0790 (Linking)
VI  - 44
IP  - 10
DP  - 2010 Nov-Dec
TI  - Gastric juice from patients "on" acid suppressive therapy can still provoke a 
      significant inflammatory reaction by human bronchial epithelial cells.
PG  - e230-5
LID - 10.1097/MCG.0b013e3181d47dc4 [doi]
AB  - BACKGROUND: Patients with reflux-related respiratory symptoms are frequently 
      treated with proton pump inhibitors (PPI). It is unclear whether aspiration of 
      gastric juice (GJ) from patients "on" PPI can provoke a similar bronchial 
      inflammatory reaction than that observed in patients "off" medication. The goal 
      of this study was to evaluate the effect of GJ from patients with and without PPI 
      treatment on production of IL-8 by human primary bronchial epithelial cells 
      (PBEC). STUDY: PBEC were exposed during 24 hours to GJ (1/1000) from patients 
      "on" (n=10) and "off" (n=13) PPI and to nonacidic gastric components (pepsin and 
      bile acids). IL-8 concentration in supernatant was measured with enzyme-linked 
      immunosorbent assay. Endotoxin level in GJ samples was analyzed with a LAL assay. 
      RESULTS: Exposure of PBEC to GJ from patients "on" PPI provoked a higher 
      production of IL-8 than GJ from patients "off" PPI [279 pg/mL (36 to 498) vs. 11 
      pg/mL (9 to 27)]. A correlation was found between pH of GJ and IL-8 production 
      (r=0.659, P=0.0006). No correlation was found between IL-8 production and 
      concentration of bile acids or pepsin. Filtration (0.20 [mu]m) of GJ from 
      patients "on" PPI reduced IL-8 production. A positive correlation was found 
      between IL-8 production and endotoxin levels of GJ samples (1/1000) (r=0.654, 
      P=0.0007). CONCLUSIONS: Exposure of bronchial epithelial cells to GJ from 
      patients "on" PPI is able to induce high IL-8 production. These results suggest 
      that aspiration of GJ in patients treated with PPI might still be able to provoke 
      a significant bronchial inflammatory reaction.
FAU - Mertens, Veerle
AU  - Mertens V
AD  - Center for Gastroenterological Research, University Hospital Gasthuisberg, 
      Belgium.
FAU - Blondeau, Kathleen
AU  - Blondeau K
FAU - Vanaudenaerde, Bart
AU  - Vanaudenaerde B
FAU - Vos, Robin
AU  - Vos R
FAU - Farre, Ricard
AU  - Farre R
FAU - Pauwels, Ans
AU  - Pauwels A
FAU - Verleden, Geert
AU  - Verleden G
FAU - Van Raemdonck, Dirk
AU  - Van Raemdonck D
FAU - Dupont, Lieven
AU  - Dupont L
FAU - Sifrim, Daniel
AU  - Sifrim D
LA  - eng
GR  - G0802313/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Gastroenterol
JT  - Journal of clinical gastroenterology
JID - 7910017
RN  - 0 (Bile Acids and Salts)
RN  - 0 (CXCL8 protein, human)
RN  - 0 (Endotoxins)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Interleukin-8)
RN  - 0 (Proton Pump Inhibitors)
RN  - EC 3.4.23.1 (Pepsin A)
SB  - IM
MH  - Aged
MH  - Bile Acids and Salts/immunology
MH  - Bronchi/*immunology
MH  - Cells, Cultured
MH  - Endotoxins/immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epithelial Cells/*immunology
MH  - Female
MH  - Gastric Acid/metabolism
MH  - Gastric Juice/*immunology/metabolism
MH  - Gastric Mucosa/*drug effects/metabolism
MH  - Gastroesophageal Reflux/*drug therapy/immunology/metabolism
MH  - Gastroscopy
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Inflammation Mediators/*metabolism
MH  - Interleukin-8/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Pepsin A/immunology
MH  - Proton Pump Inhibitors/*therapeutic use
EDAT- 2010/03/11 06:00
MHDA- 2011/02/02 06:00
CRDT- 2010/03/11 06:00
PHST- 2010/03/11 06:00 [entrez]
PHST- 2010/03/11 06:00 [pubmed]
PHST- 2011/02/02 06:00 [medline]
AID - 10.1097/MCG.0b013e3181d47dc4 [doi]
PST - ppublish
SO  - J Clin Gastroenterol. 2010 Nov-Dec;44(10):e230-5. doi: 
      10.1097/MCG.0b013e3181d47dc4.