PMID- 20217563
OWN - NLM
STAT- MEDLINE
DCOM- 20100517
LR  - 20100310
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 623
DP  - 2010
TI  - Preventing immune rejection through gene silencing.
PG  - 357-71
LID - 10.1007/978-1-60761-588-0_23 [doi]
AB  - Dendritic cells (DCs) comprise a family of professional antigen-presenting cells 
      responsible for the induction of primary immune responses. DCs are also important 
      for the induction of immunological tolerance. Recent research has revealed that 
      DC maturation is associated with activation of the NF-kappaB pathway. RelB, one 
      of the five families of Rel proteins involved in the NF-kappaB pathway, plays a 
      critical role in coordinating the terminal stages of DC maturation and has the 
      ability to induce optimal Th1 T cell responses. DCs generated from mouse bone 
      marrow can be silenced using siRNA specific for the target gene. Silencing RelB 
      in DCs will result in the generation of immunoregulatory dendritic cells that 
      inhibit allogenic T cell responses. The KLH-specific T cell response should also 
      be inhibited after the RelB siRNA treatment. Furthermore, silencing the RelB gene 
      in DCs can generate regulatory T cells. Administering donor-derived 
      RelB-silencing DCs can prevent allograft rejection in murine heart 
      transplantation.
FAU - Zhang, Xusheng
AU  - Zhang X
AD  - Department of Surgery, University of Western Ontario, London, ON, Canada.
FAU - Li, Mu
AU  - Li M
FAU - Min, Wei-Ping
AU  - Min WP
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Relb protein, mouse)
RN  - 147337-75-5 (Transcription Factor RelB)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/immunology
MH  - Dendritic Cells/*immunology/metabolism
MH  - Flow Cytometry
MH  - *Gene Silencing
MH  - Heart Transplantation/*immunology
MH  - *Immune Tolerance
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - T-Lymphocytes, Regulatory/immunology
MH  - Tissue Donors
MH  - Transcription Factor RelB/genetics
EDAT- 2010/03/11 06:00
MHDA- 2010/05/18 06:00
CRDT- 2010/03/11 06:00
PHST- 2010/03/11 06:00 [entrez]
PHST- 2010/03/11 06:00 [pubmed]
PHST- 2010/05/18 06:00 [medline]
AID - 10.1007/978-1-60761-588-0_23 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2010;623:357-71. doi: 10.1007/978-1-60761-588-0_23.