PMID- 20217564
OWN - NLM
STAT- MEDLINE
DCOM- 20100517
LR  - 20171116
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 623
DP  - 2010
TI  - Topical application of siRNA targeting cutaneous dendritic cells in allergic skin 
      disease.
PG  - 373-81
LID - 10.1007/978-1-60761-588-0_24 [doi]
AB  - RNA interference is a promising method for silencing specific genes and has great 
      potential for therapeutic applications. However, the major hurdle for therapeutic 
      application is the limited stability of double-strand RNA (dsRNA) and the absence 
      of a reliable delivery method to target cells. Skin appears to be a favorable 
      target for small interfering RNA (siRNA) therapy. Dendritic cells (DCs) exist in 
      the skin and mucosae on the front lines of defense; these cells capture antigens 
      and play a crucial role in inducing immunity and tolerance.In our recent work, we 
      have shown a successful treatment using CD86 siRNA targeting cutaneous DCs. A 
      costimulatory molecule, CD86, is induced on DCs in situ after antigen uptake, and 
      CD86-expressing DCs migrate to the regional lymph nodes to present antigens to T 
      cells. Topical application of cream-emulsified CD86 siRNA ameliorated the 
      clinical manifestations in murine contact hypersensitivity (CH) and atopic 
      dermatitis (AD)-like disease. Our method may be advantageous for the treatment of 
      allergic skin diseases.
FAU - Azuma, Miyuki
AU  - Azuma M
AD  - Department of Molecular Immunology, Graduate School, Tokyo Medical and Dental 
      University, Tokyo, Japan.
FAU - Ritprajak, Patcharee
AU  - Ritprajak P
FAU - Hashiguchi, Masaaki
AU  - Hashiguchi M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (B7-2 Antigen)
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - Administration, Topical
MH  - Animals
MH  - B7-2 Antigen/immunology
MH  - Female
MH  - Hypersensitivity/*genetics/immunology/*therapy
MH  - Langerhans Cells/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - RNA Interference
MH  - RNA, Small Interfering/*administration & dosage
EDAT- 2010/03/11 06:00
MHDA- 2010/05/18 06:00
CRDT- 2010/03/11 06:00
PHST- 2010/03/11 06:00 [entrez]
PHST- 2010/03/11 06:00 [pubmed]
PHST- 2010/05/18 06:00 [medline]
AID - 10.1007/978-1-60761-588-0_24 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2010;623:373-81. doi: 10.1007/978-1-60761-588-0_24.