PMID- 20218056
OWN - NLM
STAT- MEDLINE
DCOM- 20100406
LR  - 20100311
IS  - 0031-2983 (Print)
IS  - 0031-2983 (Linking)
VI  - 101
IP  - 5
DP  - 2009 Oct
TI  - A fluorescence in situ hybridization (FISH) procedure to assist in 
      differentiating benign from malignant melanocytic lesions.
PG  - 169-74
AB  - OBJECTIVE: Misdiagnosis of melanocytic lesions can result in unnecessary 
      psychological distress to patients, under- or overtreatment, inaccurate prognosis 
      and improper follow-up and family member surveillance. It is well recognized 
      that, despite many attempts to 1) put forth a set of histologic criteria that can 
      accurately and reproducibly be used to diagnose melanocytic lesions, and 2) 
      identify reliable markers of malignancy as an adjunct to routine histopathology, 
      misdiagnoses still occur in a significant number of cases. METHOD: A multi-color 
      FISH probe mixture has been devised to assist pathologists in differential 
      diagnosis of difficult melanocytic lesions. The mixture includes a centromeric 
      probe for chromosome 6 and unique sequence probes for three other chromosomal 
      regions that have most frequently shown amplifications or deletions in melanoma. 
      We have carried out a preliminary evaluation of this new probe set in 25 cases of 
      benign and malignant pigmented lesions. RESULTS: The tool reliably identified all 
      nevi and ordinary melanomas, and only failed to identify a pigmented epithelioid 
      melanocytoma and two malignant lesions that, by morphology and behavior, have 
      distinct features from common invasive melanomas, i.e., a desmoplastic melanoma 
      and a nevoid melanoma. Considering this, 100% specificity and 75% sensitivity was 
      achieved. CONCLUSION: The FISH tool used in this study was able to separate 
      accurately benign nevi from ordinary melanoma. Failure to identify uncommon 
      melanocytic lesions adds to its advantage and calls for further studies to unveil 
      the molecular profile of these rare entities.
FAU - Clemente, C
AU  - Clemente C
AD  - Pathology and Cytopathology Unit, Casa di Cura San Pio X, Milan, Italy.
FAU - Bettio, D
AU  - Bettio D
FAU - Venci, A
AU  - Venci A
FAU - Scopsi, L
AU  - Scopsi L
FAU - Rao, S
AU  - Rao S
FAU - Ferrari, A
AU  - Ferrari A
FAU - Piris, A
AU  - Piris A
FAU - Mihm, M C Jr
AU  - Mihm MC Jr
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Pathologica
JT  - Pathologica
JID - 0401123
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Child
MH  - Diagnosis, Differential
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Male
MH  - Melanoma/*diagnosis/genetics
MH  - Middle Aged
MH  - Nevus, Pigmented/*diagnosis/genetics
MH  - Sensitivity and Specificity
MH  - Skin Neoplasms/*diagnosis/genetics
EDAT- 2010/03/12 06:00
MHDA- 2010/04/07 06:00
CRDT- 2010/03/12 06:00
PHST- 2010/03/12 06:00 [entrez]
PHST- 2010/03/12 06:00 [pubmed]
PHST- 2010/04/07 06:00 [medline]
PST - ppublish
SO  - Pathologica. 2009 Oct;101(5):169-74.