PMID- 20219468
OWN - NLM
STAT- MEDLINE
DCOM- 20100429
LR  - 20181113
IS  - 1872-7905 (Electronic)
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 356
IP  - 1-2
DP  - 2010 Apr 30
TI  - Using carbon magnetic nanoparticles to target, track, and manipulate dendritic 
      cells.
PG  - 47-59
LID - 10.1016/j.jim.2010.02.009 [doi]
AB  - Dendritic cells (DCs) are crucial in the initiation of immune responses and are 
      primary targets in vaccination. Here, we describe fluorescent, carbon magnetic 
      nanoparticles (CMNPs) within the 20-80 nm size range that are non-toxic and 
      preferentially endocytosed by DCs. These attributes allow for DC tracing in 
      vitro, ex vivo and in vivo, by both fluorescence and MRI. We show that CMNPs 
      conjugated with an array of proteins are able to induce strong immune responses 
      in mice. The addition of TLR ligand, CpG, to the CMNPs along with protein results 
      in both T cell activation, but also a selective IFNgamma response. The magnetism 
      afforded by the CMNPs facilitates a simple DC enrichment ex vivo by magnetic 
      means from both secondary lymphoid organs, and sites of chronic inflammation. The 
      magnetic and fluorescent properties of the CMNPs allow for visualization, 
      recovery, and potentially the facilitation of directed DC migration. These 
      particles may support more efficient immunization protocols or new diagnostic 
      assays to characterize functionalities of DCs from patients.
CI  - 2010 Elsevier B.V. All rights reserved.
FAU - Schreiber, Heidi A
AU  - Schreiber HA
AD  - University of Wisconsin Department of Pathology and Laboratory Sciences, 1300 
      University Avenue, Madison, WI 53705, USA. hschreiber@wisc.edu
FAU - Prechl, Jozsef
AU  - Prechl J
FAU - Jiang, Hongquan
AU  - Jiang H
FAU - Zozulya, Alla
AU  - Zozulya A
FAU - Fabry, Zsuzsanna
AU  - Fabry Z
FAU - Denes, Ferencz
AU  - Denes F
FAU - Sandor, Matyas
AU  - Sandor M
LA  - eng
GR  - R21-A1072638/PHS HHS/United States
GR  - R01 NS037570-09A2/NS/NINDS NIH HHS/United States
GR  - R01 NS037570-08/NS/NINDS NIH HHS/United States
GR  - R01 NS037570-10/NS/NINDS NIH HHS/United States
GR  - R01 NS037570-11/NS/NINDS NIH HHS/United States
GR  - R01 NS037570/NS/NINDS NIH HHS/United States
GR  - R01-A1048087/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100226
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 7440-44-0 (Carbon)
SB  - IM
MH  - Animals
MH  - Carbon/*chemistry
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Dendritic Cells/*chemistry/cytology/immunology
MH  - Lymphocyte Activation
MH  - *Magnetics
MH  - Mice
MH  - Mice, Transgenic
MH  - Microscopy, Electron, Scanning
MH  - Microscopy, Electron, Transmission
MH  - Nanoparticles/*chemistry/ultrastructure
MH  - T-Lymphocytes/immunology
PMC - PMC2937839
MID - NIHMS184623
EDAT- 2010/03/12 06:00
MHDA- 2010/04/30 06:00
PMCR- 2011/04/30
CRDT- 2010/03/12 06:00
PHST- 2009/11/24 00:00 [received]
PHST- 2010/02/14 00:00 [revised]
PHST- 2010/02/17 00:00 [accepted]
PHST- 2010/03/12 06:00 [entrez]
PHST- 2010/03/12 06:00 [pubmed]
PHST- 2010/04/30 06:00 [medline]
PHST- 2011/04/30 00:00 [pmc-release]
AID - S0022-1759(10)00043-8 [pii]
AID - 10.1016/j.jim.2010.02.009 [doi]
PST - ppublish
SO  - J Immunol Methods. 2010 Apr 30;356(1-2):47-59. doi: 10.1016/j.jim.2010.02.009. 
      Epub 2010 Feb 26.