PMID- 20219930
OWN - NLM
STAT- MEDLINE
DCOM- 20100504
LR  - 20240317
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 84
IP  - 10
DP  - 2010 May
TI  - Comprehensive analysis of Rhesus lymphocryptovirus microRNA expression.
PG  - 5148-57
LID - 10.1128/JVI.00110-10 [doi]
AB  - Rhesus lymphocryptovirus (rLCV) and Epstein-Barr virus (EBV) are closely related 
      gammaherpesviruses that infect and cause disease in rhesus monkeys and humans, 
      respectively. Thus, rLCV is an important model system for EBV pathogenesis. Both 
      rLCV and EBV express microRNAs (miRNAs), several conserved in sequence and 
      genomic location. We have applied deep sequencing technology to obtain an 
      inventory of rLCV miRNA expression in latently rLCV-infected monkey B cells. Our 
      data confirm the presence of all previously identified mature rLCV miRNAs and 
      have resulted in the discovery of 21 new mature miRNAs arising from previously 
      identified precursor miRNAs (pre-miRNAs), as well as two novel pre-miRNAs (rL1-34 
      and rL1-35) that together generate four new mature miRNAs. Thus, the total number 
      of rLCV-encoded pre-miRNAs is 35 and the total number of rLCV mature miRNAs is 
      68, the most of any virus examined. The exact 5' and 3' ends of all mature rLCV 
      miRNAs were pinpointed, many showing marked sequence and length heterogeneity 
      that could modulate function. We further demonstrate that rLCV mature miRNAs 
      associate with Argonaute proteins in rLCV-infected B cells.
FAU - Riley, Kasandra J-L
AU  - Riley KJ
AD  - Department of Molecular Biophysics & Biochemistry, Howard Hughes Medical 
      Institute, Yale University School of Medicine, New Haven, Connecticut 06536, USA.
FAU - Rabinowitz, Gabrielle S
AU  - Rabinowitz GS
FAU - Steitz, Joan A
AU  - Steitz JA
LA  - eng
GR  - P01 CA016038/CA/NCI NIH HHS/United States
GR  - CA16038/CA/NCI NIH HHS/United States
GR  - Howard Hughes Medical Institute/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100310
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - B-Lymphocytes/virology
MH  - Cells, Cultured
MH  - *Gene Expression Profiling
MH  - Lymphocryptovirus/*physiology
MH  - Macaca mulatta
MH  - MicroRNAs/*biosynthesis
MH  - RNA, Viral/*biosynthesis
PMC - PMC2863793
EDAT- 2010/03/12 06:00
MHDA- 2010/05/05 06:00
PMCR- 2010/11/01
CRDT- 2010/03/12 06:00
PHST- 2010/03/12 06:00 [entrez]
PHST- 2010/03/12 06:00 [pubmed]
PHST- 2010/05/05 06:00 [medline]
PHST- 2010/11/01 00:00 [pmc-release]
AID - JVI.00110-10 [pii]
AID - 0110-10 [pii]
AID - 10.1128/JVI.00110-10 [doi]
PST - ppublish
SO  - J Virol. 2010 May;84(10):5148-57. doi: 10.1128/JVI.00110-10. Epub 2010 Mar 10.