PMID- 20222461
OWN - NLM
STAT- MEDLINE
DCOM- 20110930
LR  - 20100312
IS  - 1000-3061 (Print)
IS  - 1000-3061 (Linking)
VI  - 25
IP  - 11
DP  - 2009 Nov
TI  - [Construction and immunogenicity of recombinant adenoviruses expressing Cap 
      protein of PCV2 and GP5 protein of PRRSV in mice].
PG  - 1639-45
AB  - Porcine circovirus 2 (PCV2) has been implicated as the etiological agent of 
      postweaning multisystemic wasting syndrome (PMWS). Co-infection of PCV2 and 
      porcine reproductive and respiratory syndrome virus (PRRSV) can result in severe 
      economic losses to the swine industry. In this study, we constructed the 
      recombinant adenovirus rAd-Cap-GP5 expressing Cap of PCV2 and GP5 of PRRSV. And 
      the expression of Cap and GP5 protein in the HEK-293 cells inoculated with 
      rAd-Cap-GP5 were confirmed by immunoperoxidase monolayer assay (IPMA), indirect 
      immunofluorescence assay (IFA) and Western blotting, respectively. The 
      immunogenicity of recombinant adenoviruses rAd-Cap-GP5 was examined in mice by 
      vaccination with the recombinant adenovirus. The results showed that the mice 
      could produce anti-PCV2 and PRRSV antibodies detected by indirect ELISA and virus 
      neutralization assay. It indicated that rAd-Cap-GP5 could provide humoral 
      immunity responses in mice. The recombinant adenovirus rAd-Cap-GP5 might be an 
      attractive candidate vaccine for preventing the disease associated with PCV2 and 
      PRRSV infection.
FAU - Wang, Xianwei
AU  - Wang X
AD  - Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of 
      Agriculture, Nanjing Agricultural University, Nanjing 210095, China.
FAU - Li, Yufeng
AU  - Li Y
FAU - Jiang, Ping
AU  - Jiang P
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Wu Gong Cheng Xue Bao
JT  - Sheng wu gong cheng xue bao = Chinese journal of biotechnology
JID - 9426463
RN  - 0 (Capsid Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Adenoviridae/genetics/*metabolism
MH  - Animals
MH  - Capsid Proteins/*biosynthesis/genetics/immunology
MH  - Cell Line
MH  - Circoviridae Infections/prevention & control/virology
MH  - Circovirus/*genetics/metabolism
MH  - Immunization
MH  - Mice
MH  - Porcine Reproductive and Respiratory Syndrome/prevention & control/virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/metabolism
MH  - Recombinant Fusion Proteins/biosynthesis/genetics/immunology
MH  - Swine
MH  - Transfection
MH  - Viral Envelope Proteins/*biosynthesis/genetics/immunology
EDAT- 2010/03/13 06:00
MHDA- 2011/10/01 06:00
CRDT- 2010/03/13 06:00
PHST- 2010/03/13 06:00 [entrez]
PHST- 2010/03/13 06:00 [pubmed]
PHST- 2011/10/01 06:00 [medline]
PST - ppublish
SO  - Sheng Wu Gong Cheng Xue Bao. 2009 Nov;25(11):1639-45.