PMID- 20222845
OWN - NLM
STAT- MEDLINE
DCOM- 20100907
LR  - 20181201
IS  - 1532-4184 (Electronic)
IS  - 1073-1199 (Linking)
VI  - 38
IP  - 2
DP  - 2010 Apr
TI  - Coencapsulation of oxygen carriers and glucose oxidase in polyelectrolyte complex 
      capsules for the enhancement of D-gluconic acid and delta-gluconolactone 
      production.
PG  - 90-8
LID - 10.3109/10731191003634745 [doi]
AB  - A novel encapsulated oxidative biocatalyst comprising glucose oxidase (GOD) 
      coencapsulated with oxygen carriers within polyelectrolyte complex capsules was 
      developed for the production of D-gluconic acid and delta-gluconolactone. The 
      capsules containing immobilized GOD were produced by polyelectrolyte complexation 
      with sodium alginate (SA) and cellulose sulfate (CS) as polyanions, 
      poly(methylene-co-guanidine) (PMCG) as the polycation, CaCl(2) as the gelling 
      agent and NaCl as the antigelling agent (GOD-SA-CS/PMCG capsules). 
      Poly(dimethylsiloxane) (PDMS) and an emulsion of n-dodecane (DOD) or 
      perfluorodecaline (PFD) with PDMS were used as the oxygen carriers and MnO(2) was 
      used as a hydrogen peroxide decomposition catalyst. Water-soluble PDMS was found 
      to act as both an oxygen carrier and an emulsifier of water-insoluble DOD and 
      PFD. Stable microcapsules could be produced with concentrations of up to 4% (w/w) 
      of PDMS, 10% (w/w) of DOD and PFD, and 25% (w/w) of MnO(2) in the polyanion 
      solution of SA and CS. Roughly a two-fold increase in the GOD activity from 
      21.0+/-1.1 to 38.4+/-2.0 U*g(-1) and product space-time yields (STY) from 
      44.3+/-2.0 to 83.4+/-3.4 g*H*day(-1) could be achieved utilizing coencapsulated 
      oxygen carriers compared to GOD encapsulated in the absence of oxygen carriers. 
      This enhanced production does not significantly depend on the selected oxygen 
      carrier under the conditions used in this study.
FAU - Bucko, Marek
AU  - Bucko M
AD  - Department of Glycobiotechnology, Institute of Chemistry - Center for Glycomics, 
      Slovak Academy of Sciences, Bratislava, Slovak Republic. Marek.Bucko@savba.sk
FAU - Gemeiner, Peter
AU  - Gemeiner P
FAU - Vikartovska, Alica
AU  - Vikartovska A
FAU - Mislovicova, Danica
AU  - Mislovicova D
FAU - Lacik, Igor
AU  - Lacik I
FAU - Tkac, Jan
AU  - Tkac J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Artif Cells Blood Substit Immobil Biotechnol
JT  - Artificial cells, blood substitutes, and immobilization biotechnology
JID - 9431307
RN  - 0 (9-((1-phosphonomethoxycyclopropyl)methyl)guanine)
RN  - 0 (Alginates)
RN  - 0 (Alkanes)
RN  - 0 (Capsules)
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Fluorocarbons)
RN  - 0 (Gluconates)
RN  - 0 (Hexuronic Acids)
RN  - 0 (Lactones)
RN  - 0 (Manganese Compounds)
RN  - 0 (Nylons)
RN  - 0 (Organophosphonates)
RN  - 0 (Oxides)
RN  - 0 (poly(dimethylsiloxane)-polyamide copolymer)
RN  - 11A386X1QH (n-dodecane)
RN  - 54A06VV62N (perfluorodecalin)
RN  - 5Z93L87A1R (Guanine)
RN  - 8A5D83Q4RW (Glucuronic Acid)
RN  - 9004-34-6 (Cellulose)
RN  - 9032-43-3 (cellulose sulfate)
RN  - EC 1.1.3.4 (Glucose Oxidase)
RN  - R4R8J0Q44B (gluconic acid)
RN  - S88TT14065 (Oxygen)
RN  - TF219GU161 (manganese dioxide)
RN  - WQ29KQ9POT (beta-glucono-1,5-lactone)
SB  - IM
MH  - Alginates/chemistry/metabolism
MH  - Alkanes/metabolism
MH  - Biocatalysis
MH  - Capsules/chemistry/*metabolism
MH  - Cellulose/analogs & derivatives/chemistry/metabolism
MH  - Dimethylpolysiloxanes/chemistry/metabolism
MH  - *Drug Compounding
MH  - Fluorocarbons/metabolism
MH  - Gluconates/chemistry/*metabolism
MH  - Glucose Oxidase/chemistry/*metabolism
MH  - Glucuronic Acid/chemistry/metabolism
MH  - Guanine/analogs & derivatives/chemistry
MH  - Hexuronic Acids/chemistry/metabolism
MH  - Lactones/*metabolism
MH  - Manganese Compounds/metabolism
MH  - Nylons/chemistry/metabolism
MH  - Organophosphonates/chemistry
MH  - Oxides/metabolism
MH  - Oxygen/metabolism
MH  - Solubility
EDAT- 2010/03/13 06:00
MHDA- 2010/09/08 06:00
CRDT- 2010/03/13 06:00
PHST- 2010/03/13 06:00 [entrez]
PHST- 2010/03/13 06:00 [pubmed]
PHST- 2010/09/08 06:00 [medline]
AID - 10.3109/10731191003634745 [doi]
PST - ppublish
SO  - Artif Cells Blood Substit Immobil Biotechnol. 2010 Apr;38(2):90-8. doi: 
      10.3109/10731191003634745.