PMID- 20225199
OWN - NLM
STAT- MEDLINE
DCOM- 20100525
LR  - 20191210
IS  - 1934-662X (Print)
IS  - 1934-662X (Linking)
VI  - 118
IP  - 2
DP  - 2010 Apr 25
TI  - Detection of chromosomal anomalies in endometrial atypical hyperplasia and 
      carcinoma by using fluorescence in situ hybridization.
PG  - 97-104
LID - 10.1002/cncy.20072 [doi]
AB  - BACKGROUND: Endometrial cancer is the most common pelvic gynecological 
      malignancy. The diagnosis of well-differentiated endometrial adenocarcinoma, 
      atypical hyperplasia, and hyperplasia is often challenging. The authors sought to 
      investigate the utility of chromosomal anomalies for the detection of endometrial 
      hyperplasia and carcinoma using multitarget fluorescence in situ hybridization 
      (FISH). METHODS: Samples were collected by endometrial Tao brush and processed by 
      liquid-based cytological preparation protocol from consecutive cases to include 
      50 benign, 50 hyperplasia without atypia, 47 atypical hyperplasia, and 53 
      endometrial cancers. Each was hybridized using fluorescence-labeled DNA probes to 
      chromosomes 1, 8, and 10. The FISH signals were enumerated in 100 cells per case, 
      and the chromosomal anomalies were correlated with pathologic findings, including 
      histologic diagnoses on matched endometrial tissue samples. RESULTS: Numeric 
      chromosomal anomalies were found in 0% (0 of 50) of benign, 20% (10 of 50) of 
      hyperplasia, 74% (35 of 47) of atypical hyperplasia, and 87% (46 of 53) of 
      carcinoma specimens. The mean percentage of cells with chromosomal changes was 
      55% in cancer specimens, which was significantly higher than that in hyperplasia 
      without atypia (13%, P < .0001) and atypical hyperplasia (32%, P = .003). The 
      most frequent chromosomal anomaly was gain of chromosome 1. FISH anomalies had an 
      overall sensitivity of 81% and specificity of 90% for the detection of atypical 
      hyperplasia and/or endometrial carcinoma. There was no association with grade of 
      endometrial carcinoma. CONCLUSIONS: Multitarget FISH appears to be useful for the 
      differential diagnosis of hyperplasia, atypical hyperplasia, and endometrial 
      adenocarcinoma, with a high level of sensitivity and specificity. It is also a 
      potential tool for the early detection of neoplastic cells in endometrial 
      cytology specimens. Endometrial hyperplasia with FISH-detected chromosomal 
      anomalies may represent a clinically significant subset of cases that warrant 
      close clinical follow-up.
CI  - (c) 2010 American Cancer Society.
FAU - Qian, Junqi
AU  - Qian J
AD  - Bostwick Laboratories, 4355 Innslake Drive, Glen Allen, VA 23060, USA. 
      jqian@bostwicklaboratories.com
FAU - Weber, Deena
AU  - Weber D
FAU - Cochran, Richard
AU  - Cochran R
FAU - Hossain, Deloar
AU  - Hossain D
FAU - Bostwick, David G
AU  - Bostwick DG
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - Cancer Cytopathol
JT  - Cancer cytopathology
JID - 101499453
SB  - IM
MH  - Adenocarcinoma/*genetics
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 1
MH  - Chromosomes, Human, Pair 10
MH  - Chromosomes, Human, Pair 8
MH  - Endometrial Hyperplasia/diagnosis/*genetics
MH  - Endometrial Neoplasms/diagnosis/*genetics
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Sensitivity and Specificity
EDAT- 2010/03/13 06:00
MHDA- 2010/05/26 06:00
CRDT- 2010/03/13 06:00
PHST- 2010/03/13 06:00 [entrez]
PHST- 2010/03/13 06:00 [pubmed]
PHST- 2010/05/26 06:00 [medline]
AID - 10.1002/cncy.20072 [doi]
PST - ppublish
SO  - Cancer Cytopathol. 2010 Apr 25;118(2):97-104. doi: 10.1002/cncy.20072.