PMID- 20226739
OWN - NLM
STAT- MEDLINE
DCOM- 20100609
LR  - 20100511
IS  - 1521-7035 (Electronic)
IS  - 1521-6616 (Linking)
VI  - 135
IP  - 3
DP  - 2010 Jun
TI  - Dendritic/pancreatic carcinoma fusions for clinical use: Comparative functional 
      analysis of healthy- versus patient-derived fusions.
PG  - 384-400
LID - 10.1016/j.clim.2010.02.003 [doi]
AB  - Fetal calf serum (FCS)-independent pancreatic cancer cells were established in 
      plasma protein fraction (PPF)-supplemented medium that is an agent of good 
      manufacturing practice (GMP) grade. Dendritic cells (DCs) were activated with the 
      Toll-like receptor agonist, penicillin-inactivated Streptococcus pyogenes 
      (OK-432) that is also a GMP grade agent. Therefore, sufficient amounts of 
      FCS-independent fusions were successfully generated with decreased potential 
      hazards of FCS. The FCS-independent fusions expressed tumor-associated antigens, 
      HLA-DR, costimulatory molecules, IL-12, and IL-10. Stimulation of T cells with 
      fusions from healthy donors resulted in proliferation of T cells with high 
      expression levels of perforin/granzyme B and IFN-gamma and efficient induction of 
      antigen-specific cytotoxic T lymphocytes (CTLs). Selection and expansion of 
      T-cell clones were confirmed by TCR Vbeta analysis. However, fusions from 
      patients with metastatic pancreatic cancer induced increased expression levels of 
      TGF-beta1 in CD4+ CD25high T cells and low levels of CTLs with decreased 
      IFN-gamma production.
CI  - Copyright 2010 Elsevier Inc. All rights reserved.
FAU - Koido, Shigeo
AU  - Koido S
AD  - Division of Gastroenterology and Hepatology, Department of Internal Medicine, The 
      Jikei University School of Medicine, Tokyo, Japan. shigeo_koido@jikei.ac.jp
FAU - Hara, Eiichi
AU  - Hara E
FAU - Homma, Sadamu
AU  - Homma S
FAU - Namiki, Yoshihisa
AU  - Namiki Y
FAU - Komita, Hideo
AU  - Komita H
FAU - Takahara, Akitaka
AU  - Takahara A
FAU - Nagasaki, Eijiro
AU  - Nagasaki E
FAU - Ito, Masaki
AU  - Ito M
FAU - Sagawa, Yukiko
AU  - Sagawa Y
FAU - Mitsunaga, Makoto
AU  - Mitsunaga M
FAU - Uchiyama, Kan
AU  - Uchiyama K
FAU - Satoh, Kenichi
AU  - Satoh K
FAU - Arihiro, Seiji
AU  - Arihiro S
FAU - Ohkusa, Toshifumi
AU  - Ohkusa T
FAU - Gong, Jianlin
AU  - Gong J
FAU - Tajiri, Hisao
AU  - Tajiri H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100311
PL  - United States
TA  - Clin Immunol
JT  - Clinical immunology (Orlando, Fla.)
JID - 100883537
RN  - 0 (Antigens, Neoplasm)
SB  - IM
MH  - Antigens, Neoplasm/immunology/metabolism
MH  - Cell Fusion/methods
MH  - Cell Separation
MH  - Cells, Cultured
MH  - Dendritic Cells/*cytology/*immunology
MH  - Flow Cytometry
MH  - Humans
MH  - Hybrid Cells/*immunology
MH  - Immunotherapy/*methods
MH  - Lymphocyte Activation/immunology
MH  - Pancreatic Neoplasms/*immunology
MH  - T-Lymphocyte Subsets/immunology
MH  - T-Lymphocytes/immunology
EDAT- 2010/03/17 06:00
MHDA- 2010/06/10 06:00
CRDT- 2010/03/16 06:00
PHST- 2009/09/08 00:00 [received]
PHST- 2010/02/06 00:00 [revised]
PHST- 2010/02/09 00:00 [accepted]
PHST- 2010/03/16 06:00 [entrez]
PHST- 2010/03/17 06:00 [pubmed]
PHST- 2010/06/10 06:00 [medline]
AID - S1521-6616(10)00044-6 [pii]
AID - 10.1016/j.clim.2010.02.003 [doi]
PST - ppublish
SO  - Clin Immunol. 2010 Jun;135(3):384-400. doi: 10.1016/j.clim.2010.02.003. Epub 2010 
      Mar 11.