PMID- 20228543
OWN - NLM
STAT- MEDLINE
DCOM- 20100923
LR  - 20151119
IS  - 0973-3922 (Electronic)
IS  - 0378-6323 (Linking)
VI  - 76
IP  - 2
DP  - 2010 Mar-Apr
TI  - Anti-nucleosome antibodies as a disease marker in systemic lupus erythematosus 
      and its correlation with disease activity and other autoantibodies.
PG  - 145-9
LID - 10.4103/0378-6323.60558 [doi]
AB  - BACKGROUND: Detection of anti-nucleosome antibodies (anti-nuc) in patients with 
      systemic lupus erythematosus (SLE) has been well established and it is claimed 
      that their presence is associated with disease activity. AIMS: The aim of this 
      study is to evaluate the incidence of anti-nuc antibodies and to correlate them 
      with disease activity and its association with other autoantibodies like 
      anti-nuclear antibodies (ANA), anti-double stranded DNA (anti-dsDNA), 
      anti-histone antibodies (AHA), as well as autoantibodies to histone subfractions 
      like H1, (H2A-H4) complex, H2B, and H3. METHODS: This cross-sectional study 
      included 100 SLE patients referred from the Rheumatology, Dermatology, and 
      Nephrology Departments. SLE disease activity was evaluated by using SLE-Disease 
      Activity Index (SLEDAI) score. A patient was defined as having active SLE when 
      the SLEDAI score was more than 5.0. Fifty normal controls were also tested as a 
      healthy control group. Anti-nuc antibodies, anti-dsDNA, and AHA were tested by 
      Enzyme-Linked Immunosorbent Assay (ELISA) and ANA was detected by an indirect 
      immunofluorescence test. RESULTS: All patients studied were in an active stage of 
      disease and were untreated, of which 44 patients had renal biopsy-proven kidney 
      involvement, which was categorized as lupus nephritis (LN) and 56 patients did 
      not show any renal manifestations (SLE without LN). Anti-nuc antibodies were 
      positive in 88%, anti-dsDNA in 80%, and AHA in 38% of the cases. ANA was positive 
      in all SLE patients studied. None of the normal controls was found to be positive 
      for these antibodies. Although a slightly higher incidence of autoantibodies were 
      noted in LN, there was no statistical difference noted between LN and SLE without 
      LN groups for anti-nuc and anti-dsDNA antibodies (p > 0.05). A higher incidence 
      of autoantibodies to ANA specificities were noted in anti-nuc positive cases, but 
      there was no statistical difference between anti-nuc positive and anti-nuc 
      negative cases for ANA specificities among LN and SLE without nephritis groups (p 
      > 0.05). CONCLUSIONS: Anti-nuc antibody detection could be a better tool for the 
      diagnosis of SLE. Although there was no significant difference in LN and SLE 
      without LN groups, this study suggests that anti-nuc detection can be useful as 
      an additional disease activity marker to other laboratory tests.
FAU - Pradhan, Vandana D
AU  - Pradhan VD
AD  - Department of Autoimmune Disorders, National Institute of Immunohematology, 
      Indian Council of Medical Research, 13th floor, KEM Hospital, Parel, Mumbai, 
      India.
FAU - Patwardhan, Manisha M
AU  - Patwardhan MM
FAU - Ghosh, Kanjaksha
AU  - Ghosh K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Indian J Dermatol Venereol Leprol
JT  - Indian journal of dermatology, venereology and leprology
JID - 7701852
RN  - 0 (Antibodies, Antinuclear)
RN  - 0 (Biomarkers)
RN  - 0 (Nucleosomes)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antibodies, Antinuclear/biosynthesis/*blood
MH  - Biomarkers/blood
MH  - Cross-Sectional Studies
MH  - Double-Blind Method
MH  - Female
MH  - Humans
MH  - Lupus Erythematosus, Systemic/diagnosis/*immunology
MH  - Male
MH  - Nucleosomes/*immunology/metabolism
MH  - Young Adult
EDAT- 2010/03/17 06:00
MHDA- 2010/09/24 06:00
CRDT- 2010/03/16 06:00
PHST- 2010/03/16 06:00 [entrez]
PHST- 2010/03/17 06:00 [pubmed]
PHST- 2010/09/24 06:00 [medline]
AID - ijdvl_2010_76_2_145_60558 [pii]
AID - 10.4103/0378-6323.60558 [doi]
PST - ppublish
SO  - Indian J Dermatol Venereol Leprol. 2010 Mar-Apr;76(2):145-9. doi: 
      10.4103/0378-6323.60558.