PMID- 20228932
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20200930
IS  - 2092-6685 (Electronic)
IS  - 1598-2629 (Print)
IS  - 1598-2629 (Linking)
VI  - 10
IP  - 1
DP  - 2010 Feb
TI  - Effects of IL-3 and SCF on Histamine Production Kinetics and Cell Phenotype in 
      Rat Bone Marrow-derived Mast Cells.
PG  - 15-25
LID - 10.4110/in.2010.10.1.15 [doi]
AB  - BACKGROUND: Rat mast cells were regarded as a good model for mast cell function 
      in immune response. METHODS: Rat bone marrow mast cells (BMMC) were prepared both 
      by recombinant rat IL-3 (rrIL-3) and by recombinant mouse stem cell factor 
      (rmSCF), and investigated for both proliferation and differentiation in time 
      course. Rat BMMC was induced by culture of rat bone marrow cells (BMCs) in the 
      presence of both rrIL-3 (5 ng/ml) and rmSCF (5 ng/ml). Culture media were changed 
      2 times per week with the cell number condition of 5x10(4)/ml in 6 well plate. 
      Proliferation was analyzed by cell number and cell counting kit-8 (CCK-8) and 
      differentiation was by rat mast cell protease (RMCP) II and histamine. RESULTS: 
      Cell proliferation rates reached a maximum at 8 or 11 days of culture and 
      decreased thereafter. However, both RMCP II production and histamine synthesis 
      peaked after 11 days of culture. By real time RT-PCR, the level of histidine 
      decarboxylase mRNA was more than 500 times higher on culture day 11 than on 
      culture day 5. By transmission electron microscopy, the cells were heterogeneous 
      in size and contained cytoplasmic granules. Using gated flow cytometry, we showed 
      that cultured BMCs expressed high levels of FcepsilonRI and the mast cell 
      antigen, ganglioside, on culture day 11. CONCLUSION: These results indicate that 
      rat BMMCs were generated by culturing BMCs in the presence of rrIL-3 and rmSCF 
      and that the BMMCs have the characteristics of mucosal mast cells.
FAU - Lee, Haneul Nari
AU  - Lee HN
AD  - Department of Microbiology and Immunology, Korea University Graduate School, 
      Seoul 136-705, Korea.
FAU - Kim, Chul Hwan
AU  - Kim CH
FAU - Song, Gwan Gyu
AU  - Song GG
FAU - Cho, Sung-Weon
AU  - Cho SW
LA  - eng
PT  - Journal Article
DEP - 20100228
PL  - Korea (South)
TA  - Immune Netw
JT  - Immune network
JID - 101137270
PMC - PMC2837153
OTO - NOTNLM
OT  - Bone marrow
OT  - IL-3
OT  - Mast cell
OT  - Rat
OT  - Stem cell factor
COIS- The authors declare no financial or commercial conflicts of interest.
EDAT- 2010/03/17 06:00
MHDA- 2010/03/17 06:01
PMCR- 2010/02/01
CRDT- 2010/03/16 06:00
PHST- 2009/12/14 00:00 [received]
PHST- 2009/12/29 00:00 [revised]
PHST- 2010/01/25 00:00 [accepted]
PHST- 2010/03/16 06:00 [entrez]
PHST- 2010/03/17 06:00 [pubmed]
PHST- 2010/03/17 06:01 [medline]
PHST- 2010/02/01 00:00 [pmc-release]
AID - 10.4110/in.2010.10.1.15 [doi]
PST - ppublish
SO  - Immune Netw. 2010 Feb;10(1):15-25. doi: 10.4110/in.2010.10.1.15. Epub 2010 Feb 
      28.