PMID- 20231497
OWN - NLM
STAT- MEDLINE
DCOM- 20100413
LR  - 20100316
IS  - 1538-3652 (Electronic)
IS  - 0003-987X (Linking)
VI  - 146
IP  - 3
DP  - 2010 Mar
TI  - Sensitivity of fluorescence in situ hybridization for melanoma diagnosis using 
      RREB1, MYB, Cep6, and 11q13 probes in melanoma subtypes.
PG  - 273-8
LID - 10.1001/archdermatol.2009.386 [doi]
AB  - OBJECTIVE: To evaluate the diagnostic sensitivity of fluorescence in situ 
      hybridization (FISH) using probes targeting 6p25, 6q23, 11q13, and Cep6 in 
      melanoma subtypes. DESIGN: Blinded comparison of chromosomal copy number changes 
      detected using FISH targeting 6p25, 6q23, 11q13, and Cep6 in benign nevi and 
      melanoma subtypes. SETTING: Dermatopathology Laboratory, Department of 
      Dermatology, Northwestern University, Chicago, Illinois. PARTICIPANTS: One 
      hundred ten individuals with benign nevi and 123 with melanoma (70 superficial 
      spreading, 28 lentigo maligna, 22 nodular, and 3 acral lentiginous melanomas). 
      MAIN OUTCOME MEASURES: Sensitivity of previously developed criteria using FISH 
      using probes targeting 6p25, 6q23, 11q13, and Cep6 in the melanoma subtypes. 
      RESULTS: Overall, sensitivity was 83.0% and specificity was 94.0%. The 6p25 gain 
      criterion had the highest sensitivity overall and in each subtype. The assay was 
      most sensitive in the subgroups of nodular and acral melanomas and least 
      sensitive in the superficial spreading subtype. The 11q13 gain was more commonly 
      seen in chronically sun-damaged skin and infrequently in non-chronically 
      sun-damaged skin. CONCLUSIONS: Heterogeneous changes in melanoma occur at the 
      molecular level, and the changes are different among melanoma subtypes. Clonal 
      abnormalities in chromosome 6 with increased copies of the short arm relative to 
      the long arm are common in all melanoma subtypes, suggesting that isochromosome 6 
      is common in all variants of cutaneous melanoma subtypes. An increase in copy 
      number of 11q13 is most frequent in chronically sun-damaged melanomas.
FAU - Gerami, Pedram
AU  - Gerami P
AD  - Department of Dermatology, Feinberg School of Medicine, Northwestern University, 
      676 N St Clair Street, Chicago, IL 60611, USA. Pedram.Gerami@nmff.org
FAU - Mafee, Mariam
AU  - Mafee M
FAU - Lurtsbarapa, Teekay
AU  - Lurtsbarapa T
FAU - Guitart, Joan
AU  - Guitart J
FAU - Haghighat, Zahra
AU  - Haghighat Z
FAU - Newman, Marissa
AU  - Newman M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arch Dermatol
JT  - Archives of dermatology
JID - 0372433
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Proto-Oncogene Proteins c-myb)
RN  - 0 (RREB1 protein, human)
RN  - 0 (Transcription Factors)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Aged
MH  - Chromosome Aberrations
MH  - Chromosomes, Human, Pair 11
MH  - Chromosomes, Human, Pair 6
MH  - Cyclin D1/*genetics
MH  - DNA Probes
MH  - DNA, Neoplasm/*analysis
MH  - DNA-Binding Proteins/*genetics
MH  - Diagnosis, Differential
MH  - Female
MH  - Genetic Predisposition to Disease
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Melanoma/*diagnosis/genetics
MH  - Middle Aged
MH  - Proto-Oncogene Proteins c-myb/*genetics
MH  - Sensitivity and Specificity
MH  - Skin Neoplasms/*diagnosis/genetics
MH  - Transcription Factors/*genetics
MH  - Zinc Fingers
EDAT- 2010/03/17 06:00
MHDA- 2010/04/14 06:00
CRDT- 2010/03/17 06:00
PHST- 2010/03/17 06:00 [entrez]
PHST- 2010/03/17 06:00 [pubmed]
PHST- 2010/04/14 06:00 [medline]
AID - 146/3/273 [pii]
AID - 10.1001/archdermatol.2009.386 [doi]
PST - ppublish
SO  - Arch Dermatol. 2010 Mar;146(3):273-8. doi: 10.1001/archdermatol.2009.386.