PMID- 20233419
OWN - NLM
STAT- MEDLINE
DCOM- 20100503
LR  - 20181113
IS  - 1471-2164 (Electronic)
IS  - 1471-2164 (Linking)
VI  - 11
DP  - 2010 Mar 16
TI  - Characterization of the ovine ribosomal protein SA gene and its pseudogenes.
PG  - 179
LID - 10.1186/1471-2164-11-179 [doi]
AB  - BACKGROUND: The ribosomal protein SA (RPSA), previously named 37-kDa laminin 
      receptor precursor/67-kDa laminin receptor (LRP/LR) is a multifunctional protein 
      that plays a role in a number of pathological processes, such as cancer and prion 
      diseases. In all investigated species, RPSA is a member of a multicopy gene 
      family consisting of one full length functional gene and several pseudogenes. 
      Therefore, for studies on RPSA related pathways/pathologies, it is important to 
      characterize the whole family and to address the possible function of the other 
      RPSA family members. The present work aims at deciphering the RPSA family in 
      sheep. RESULTS: In addition to the full length functional ovine RPSA gene, 11 
      other members of this multicopy gene family, all processed pseudogenes, were 
      identified. Comparison between the RPSA transcript and these pseudogenes shows a 
      large variety in sequence identities ranging from 99% to 74%. Only one of the 11 
      pseudogenes, i.e. RPSAP7, shares the same open reading frame (ORF) of 295 amino 
      acids with the RPSA gene, differing in only one amino acid. All members of the 
      RPSA family were annotated by comparative mapping and fluorescence in situ 
      hybridization (FISH) localization. Transcription was investigated in the 
      cerebrum, cerebellum, spleen, muscle, lymph node, duodenum and blood, and 
      transcripts were detected for 6 of the 11 pseudogenes in some of these tissues. 
      CONCLUSIONS: In the present work we have characterized the ovine RPSA family. Our 
      results have revealed the existence of 11 ovine RPSA pseudogenes and provide new 
      data on their structure and sequence. Such information will facilitate molecular 
      studies of the functional RPSA gene taking into account the existence of these 
      pseudogenes in the design of experiments. It remains to be investigated if the 
      transcribed members are functional as regulatory non-coding RNA or as functional 
      proteins.
FAU - Van den Broeke, Alice
AU  - Van den Broeke A
AD  - Department of Nutrition, Genetics and Ethology, Faculty of Veterinary Medicine, 
      Ghent University, Heidestraat 19, Merelbeke, Belgium.
FAU - Van Poucke, Mario
AU  - Van Poucke M
FAU - Marcos-Carcavilla, Ane
AU  - Marcos-Carcavilla A
FAU - Hugot, Karine
AU  - Hugot K
FAU - Hayes, Helene
AU  - Hayes H
FAU - Bertaud, Maud
AU  - Bertaud M
FAU - Van Zeveren, Alex
AU  - Van Zeveren A
FAU - Peelman, Luc J
AU  - Peelman LJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100316
PL  - England
TA  - BMC Genomics
JT  - BMC genomics
JID - 100965258
RN  - 0 (Receptors, Laminin)
RN  - 0 (Ribosomal Proteins)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Chromosomes, Artificial, Bacterial
MH  - Contig Mapping
MH  - Gene Expression Profiling
MH  - Gene Library
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Multigene Family
MH  - Open Reading Frames
MH  - *Pseudogenes
MH  - Receptors, Laminin/*genetics
MH  - Ribosomal Proteins/*genetics
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Tagged Sites
MH  - Sheep, Domestic/*genetics
PMC - PMC2850357
EDAT- 2010/03/18 06:00
MHDA- 2010/05/04 06:00
PMCR- 2010/03/16
CRDT- 2010/03/18 06:00
PHST- 2009/12/09 00:00 [received]
PHST- 2010/03/16 00:00 [accepted]
PHST- 2010/03/18 06:00 [entrez]
PHST- 2010/03/18 06:00 [pubmed]
PHST- 2010/05/04 06:00 [medline]
PHST- 2010/03/16 00:00 [pmc-release]
AID - 1471-2164-11-179 [pii]
AID - 10.1186/1471-2164-11-179 [doi]
PST - epublish
SO  - BMC Genomics. 2010 Mar 16;11:179. doi: 10.1186/1471-2164-11-179.