PMID- 20233702
OWN - NLM
STAT- MEDLINE
DCOM- 20100824
LR  - 20190923
IS  - 0253-9772 (Print)
IS  - 0253-9772 (Linking)
VI  - 32
IP  - 3
DP  - 2010 Mar
TI  - [Screening of microsatellite markers associated with cold tolerance of large 
      yellow croaker (Pseudosciaena crocea R.)].
PG  - 248-53
AB  - Cold tolerance is one of the major economic characters in fish. In order to 
      discuss the cold tolerance of large yellow croaker (Pseudosciaena crocea R.), 
      fifteen fluorescent dye-labeled microsatellite markers were applied to detect 
      genetic differences between F1 offsprings of cold tolerance group and normal 
      group of large yellow croaker by SSR-PCR. Each group contained 20 randomly and 
      separately sampled individuals. As a result, marker LYC0002 five alleles 
      (LYC0002(104 bp), LYC0002(106 bp), LYC0002(108 bp), LYC0002(110 bp), and 
      LYC0002(112 bp)) were amplified with marker LYC0002 in both groups and 60% 
      (12/20) of individuals had allele LYC0002(112 bp) in cold tolerance group 
      exclusively, which indicated that this allele is probably sensitive to 
      temperature and associated with gene for cold tolerance. In addition, four 
      alleles (LYC0002(106 bp), LYC0002(108 bp), LYC0002(110 bp), and LYC0002(112 bp)) 
      were sequenced individually. Sequence alignments showed that LYC0002(112 bp) 
      allele contains 10 (CA) repeats, the remaining three alleles lacked one (CA) one 
      by one, corresponding to the stepwise mutation model (SMM) of microsatellite.
FAU - Gao, Guo-Qiang
AU  - Gao GQ
AD  - Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Harbin 150070, China. gqgao2008@126.com
FAU - Chang, Yu-Mei
AU  - Chang YM
FAU - Han, Qi-Xia
AU  - Han QX
FAU - Chi, Bing-Jie
AU  - Chi BJ
FAU - Li, Ming-Yun
AU  - Li MY
FAU - Xue, Liang-Yi
AU  - Xue LY
FAU - Liang, Li-Qun
AU  - Liang LQ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Yi Chuan
JT  - Yi chuan = Hereditas
JID - 9436478
SB  - IM
MH  - Animals
MH  - *Cold Temperature
MH  - Microsatellite Repeats/*genetics
MH  - Perciformes/*genetics
MH  - Polymerase Chain Reaction
EDAT- 2010/03/18 06:00
MHDA- 2010/08/25 06:00
CRDT- 2010/03/18 06:00
PHST- 2010/03/18 06:00 [entrez]
PHST- 2010/03/18 06:00 [pubmed]
PHST- 2010/08/25 06:00 [medline]
AID - 0253-9772(2010)3-248-06 [pii]
AID - 10.3724/sp.j.1005.2010.00248 [doi]
PST - ppublish
SO  - Yi Chuan. 2010 Mar;32(3):248-53. doi: 10.3724/sp.j.1005.2010.00248.