PMID- 20233704 OWN - NLM STAT- MEDLINE DCOM- 20100824 LR - 20190923 IS - 0253-9772 (Print) IS - 0253-9772 (Linking) VI - 32 IP - 3 DP - 2010 Mar TI - [Detection of maize centromeric repeats in the relatives of maize using fluorescence in situ hybridization]. PG - 264-70 AB - In order to analyze the conservation of maize centromeric satellite DNA (CentC) and centromeric retrotransposon (CRM) in the subspecies and relatives of Zea mays, dual fluorescence in situ hybridization (FISH) was used to detect the existence and distribution of the above two repetitive sequences in Zea mays ssp. mexicana, Z. diploperennis, Z. perennis, Tripsacum dactyloides, Coix lacryma-jobi, and Sorghum bicolor. In Z. mays ssp. mexicana, Z. diploperennis, and Z. perennis, both CentC and CRM probes produced strong or relatively strong signals in the centromeric regions of all chromosomes. There was an obvious variation in the intensity of hybridization signals on different chromosomes, indicating that different centromeres have different amounts of CentC and CRM sequences. In some centromeres, the intensity of CentC signals differed from that of CRM signals and was free from overlapping. In T. dactyloides, only weak CentC and CRM signals were detected in the centromeric regions of most chromosomes, while in C. lacryma-jobi and S. bicolor only relatively strong or strong CRM signals primarily located in the centromeric regions were detected. This result indicates that CentC is highly conserved among the subspecies of Z. mays and the species of Zea, and has high conservation in Tripsacum, a genus that is most closely related to Zea, and CRM is conserved among the species of grass family either closely or distantly related to Zea. FAU - She, Chao-Wen AU - She CW AD - Department of Life Sciences, Huaihua University, Huaihua 418008, China. shechaowen@tom.com FAU - Jiang, Xiang-Hui AU - Jiang XH FAU - Song, Yun-Chun AU - Song YC FAU - Liu, Wei AU - Liu W LA - chi PT - English Abstract PT - Journal Article PL - China TA - Yi Chuan JT - Yi chuan = Hereditas JID - 9436478 RN - 0 (DNA, Satellite) RN - 0 (Retroelements) SB - IM MH - Centromere/*genetics MH - DNA, Satellite/genetics MH - In Situ Hybridization, Fluorescence/*methods MH - Retroelements/genetics MH - Zea mays/*genetics EDAT- 2010/03/18 06:00 MHDA- 2010/08/25 06:00 CRDT- 2010/03/18 06:00 PHST- 2010/03/18 06:00 [entrez] PHST- 2010/03/18 06:00 [pubmed] PHST- 2010/08/25 06:00 [medline] AID - 0253-9772(2010)3-264-07 [pii] AID - 10.3724/sp.j.1005.2010.00264 [doi] PST - ppublish SO - Yi Chuan. 2010 Mar;32(3):264-70. doi: 10.3724/sp.j.1005.2010.00264.