PMID- 20236262
OWN - NLM
STAT- MEDLINE
DCOM- 20100827
LR  - 20171116
IS  - 1600-0897 (Electronic)
IS  - 1046-7408 (Linking)
VI  - 64
IP  - 1
DP  - 2010 Jul 1
TI  - Elevated NK cell cytotoxicity, CD158a expression in NK cells and activated T 
      lymphocytes in peripheral blood of women with IVF failures.
PG  - 58-67
LID - 10.1111/j.1600-0897.2010.00825.x [doi]
AB  - PROBLEM: The aim of this study was to evaluate the role of elevated natural 
      killer cytotoxicity (NKc) in women with multiple implantation failures (IF) in 
      vitro fertilization-embryo transfer (IVF-ET) cycles. METHODS OF STUDY: 
      Seventy-nine antiphospholipid antibodies-negative women with IF including 33 
      women with elevated NKc were selected for investigation. K-562 cell line was used 
      to evaluate NKc. Lymphocyte subsets, intracellular cytokines [interferon 
      (IFN)-gamma, interleukin (IL)-4, tumour necrosis factor, IL-10], expression of 
      activating markers [CD69, human leukocyte antigen (HLA)-DR], CD8, KIR (CD158a), 
      CD95, and chemokine receptors (CXCR3, CCR4) were estimated by flow cytometry. 
      RESULTS: In women with IF, levels of NKc were higher than in IVF successful 
      women. IF was associated with higher expression of CD8, CD158a, and HLA-DR in NK 
      cells, activating markers in T lymphocytes, and lower levels of CCR4+ and IL-4+ T 
      lymphocyte subsets. Predictive value of single elevated NKc for IVF success was 
      0.85, but addition of two other abnormal parameters resulted in its decrease to 
      <0.39. CONCLUSIONS: Elevated NKc is negative factor, though not critical for 
      implantation in IVF cycles. Immune mechanism of IVF failure includes not only 
      elevated NKc but also some other factors, such as elevated expression of CD8 and 
      CD158a on NK cells, T lymphocyte activation, and diminished T helper 2 
      parameters.
FAU - Chernyshov, Viktor P
AU  - Chernyshov VP
AD  - Laboratory of Immunology, Institute of Pediatrics, Obstetrics and Gynecology, 
      Academy of Medical Sciences of Ukraine, Kiev, Ukraine. chernyshov@ukr.net
FAU - Sudoma, Iryna O
AU  - Sudoma IO
FAU - Dons'koi, Boris V
AU  - Dons'koi BV
FAU - Kostyuchyk, Anton A
AU  - Kostyuchyk AA
FAU - Masliy, Yulia V
AU  - Masliy YV
LA  - eng
PT  - Journal Article
DEP - 20100317
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (CD56 Antigen)
RN  - 0 (Receptors, KIR2DL1)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adult
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - CD56 Antigen/metabolism
MH  - *Cytotoxicity, Immunologic
MH  - Embryo Implantation/*immunology
MH  - Female
MH  - *Fertilization in Vitro
MH  - Humans
MH  - Infertility, Female/blood/*immunology
MH  - Interferon-gamma/metabolism
MH  - Interleukin-10/metabolism
MH  - Interleukin-4/metabolism
MH  - Killer Cells, Natural/*immunology
MH  - Lymphocyte Activation
MH  - Middle Aged
MH  - Receptors, KIR2DL1/*metabolism
MH  - T-Lymphocyte Subsets/immunology
EDAT- 2010/03/20 06:00
MHDA- 2010/08/28 06:00
CRDT- 2010/03/19 06:00
PHST- 2010/03/19 06:00 [entrez]
PHST- 2010/03/20 06:00 [pubmed]
PHST- 2010/08/28 06:00 [medline]
AID - AJI825 [pii]
AID - 10.1111/j.1600-0897.2010.00825.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 2010 Jul 1;64(1):58-67. doi: 
      10.1111/j.1600-0897.2010.00825.x. Epub 2010 Mar 17.